Invitrogen

MCP-1 Polyclonal Antibody

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View all (67) CCL2 (MCP-1) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-46954

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)
-

Neutralization (Neu)

0.5-2.5 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant mouse CCL2/JE/MCP-1 Gln24-Arg96
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609536

Product Specific Information

In sandwich ELISAs, less than 0.01% cross-reactivity with recombinant human (rh) MCP-1, rhMCP-2, rhMCP-3, recombinant mouse (rm) MARC, rhMCP-4, rmMCP-5, rmMDC, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rmMIP-2, rmMIP-3 beta, rmRANTES, rmTARC, and rmTeck is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 2; H-MCP-1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; Small-inducible cytokine A2

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Gene Aliases: AI323594; Ccl2; HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje; SMC-CF

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UniProt ID: (Mouse) P10148

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Entrez Gene ID: (Mouse) 20296

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Function(s)
G-protein coupled receptor binding cytokine activity protein binding chemokine activity heparin binding CCR2 chemokine receptor binding
Process(es)
angiogenesis positive regulation of leukocyte mediated cytotoxicity positive regulation of endothelial cell proliferation leukocyte migration involved in inflammatory response positive regulation of leukocyte migration positive regulation of cellular extravasation cellular calcium ion homeostasis chemotaxis inflammatory response immune response cytoskeleton organization transforming growth factor beta receptor signaling pathway glial cell migration regulation of cell shape response to heat response to wounding regulation of vascular endothelial growth factor production positive regulation of macrophage chemotaxis negative regulation of angiogenesis cytokine-mediated signaling pathway positive regulation of cell-cell adhesion neutrophil chemotaxis positive regulation of tumor necrosis factor production positive regulation of collagen biosynthetic process helper T cell extravasation monocyte homeostasis positive regulation of cell adhesion vascular endothelial growth factor receptor signaling pathway eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of synaptic transmission positive regulation of T cell activation positive regulation of nitric-oxide synthase biosynthetic process chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of monocyte chemotaxis positive regulation of immune complex clearance by monocytes and macrophages positive regulation of protein targeting to membrane positive regulation of apoptotic cell clearance
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