Proteintech

MCT1 Polyclonal Antibody

MCT1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

MCT1 Antibody (20139-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 20139-1-AP (MCT1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MCT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MCT1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20139-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 33 publications 33 publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MCT1 Fusion Protein Ag14098 (451-500 aa encoded by BC026317)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EQKANEQKK ESKEEETSID VAGKPNEVTK AAESPDQKDT DGGPKEEESP V (451-500 aa encoded by BC026317)

Target Information

SLC16A1 is a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes. Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lactate transporter MCT1 in hepatic stellate cells promotes fibrotic collagen expression in nonalcoholic steatohepatitis.

bioRxiv : the preprint server for biology

Min K,Yenilmez B,Kelly M,Echeverria D,Elleby M,Lifshitz LM,Raymond N,Tsagkaraki E,Harney SM,DiMarzio C,Wang H,McHugh N,Bramato B,Morrision B,Rothstein JD,Khvorova A,Czech MP

Thu Oct 19 00:00:00 UTC 2023

Lactate transporter MCT1 in hepatic stellate cells promotes fibrotic collagen expression in nonalcoholic steatohepatitis.

bioRxiv : the preprint server for biology

Min K,Yenilmez B,Kelly M,Echeverria D,Elleby M,Lifshitz LM,Raymond N,Tsagkaraki E,Harney SM,DiMarzio C,Wang H,McHugh N,Bramato B,Morrision B,Rothstein JD,Khvorova A,Czech MP

Thu Oct 19 00:00:00 UTC 2023

Lactate transporter MCT1 in hepatic stellate cells promotes fibrotic collagen expression in nonalcoholic steatohepatitis.

bioRxiv : the preprint server for biology

Min K,Yenilmez B,Kelly M,Echeverria D,Elleby M,Lifshitz LM,Raymond N,Tsagkaraki E,Harney SM,DiMarzio C,Wang H,McHugh N,Bramato B,Morrision B,Rothstein JD,Khvorova A,Czech MP

Thu Oct 19 00:00:00 UTC 2023

Immunohistochemical evaluation and prognostic value of monocarboxylate transporter 1 (MCT1) and 4 (MCT4) in T-cell non-Hodgkin lymphoma.

Clinical and experimental medicine

Zhao H,Chen Y,Liao YP,Chen HM,Yang QH,Xiao Y,Luo J,Chen ZZ,Yi L,Hu GY

Wed Feb 01 00:00:00 UTC 2023

Functional heterogeneity of MCT1 and MCT4 in metabolic reprogramming affects osteosarcoma growth and metastasis.

Journal of orthopaedic surgery and research

Sheng G,Gao Y,Wu H,Liu Y,Yang Y

Wed Feb 22 00:00:00 UTC 2023

Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.

The Journal of biological chemistry

Xu Y,Kusuyama J,Osana S,Matsuhashi S,Li L,Takada H,Inada H,Nagatomi R

Thu Jun 01 00:00:00 UTC 2023

Hyperpolarized ¹³C-Pyruvate to Assess Response to Anti-PD1 Immune Checkpoint Inhibition in YUMMER 1.7 Melanoma Xenografts.

International journal of molecular sciences

Farah C,Neveu MA,Bouzin C,Knezevic Z,Gallez B,Leucci E,Baurain JF,Mignion L,Jordan BF

Sat Jan 28 00:00:00 UTC 2023

Lactate metabolism is essential in early-onset mitochondrial myopathy.

Science advances

Chen Z,Bordieanu B,Kesavan R,Lesner NP,Venigalla SSK,Shelton SD,DeBerardinis RJ,Mishra P

Wed Jan 04 00:00:00 UTC 2023

Arteriolosclerosis differs from venular collagenosis in relation to cerebrovascular parenchymal damages: an autopsy-based study.

Stroke and vascular neurology

Cao Y,Huang MY,Mao CH,Wang X,Xu YY,Qian XJ,Ma C,Qiu WY,Zhu YC

Tue Aug 01 00:00:00 UTC 2023

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Cell reports

Chen F,Zhu S,Kang R,Tang D,Liu J

Tue Jan 31 00:00:00 UTC 2023

Monocarboxylate transporter 1 is a novel target for breast cancer stem like-cell inhibition by diallyl trisulfide.

Molecular carcinogenesis

Kim SH,Singh SV

Mon Aug 01 00:00:00 UTC 2022

Monocarboxylate transporter 1 is a novel target for breast cancer stem like-cell inhibition by diallyl trisulfide.

Molecular carcinogenesis

Kim SH,Singh SV

Mon Aug 01 00:00:00 UTC 2022

Lactate Activates AMPK Remodeling of the Cellular Metabolic Profile and Promotes the Proliferation and Differentiation of C2C12 Myoblasts.

International journal of molecular sciences

Zhou Y,Liu X,Huang C,Lin D

Sun Nov 13 00:00:00 UTC 2022

Simultaneous targeting of glycolysis and oxidative phosphorylation as a therapeutic strategy to treat diffuse large B-cell lymphoma.

British journal of cancer

Noble RA,Thomas H,Zhao Y,Herendi L,Howarth R,Dragoni I,Keun HC,Vellano CP,Marszalek JR,Wedge SR

Thu Sep 01 00:00:00 UTC 2022

Limosilactobacillus reuteri and caffeoylquinic acid synergistically promote adipose browning and ameliorate obesity-associated disorders.

Microbiome

Liu Y,Zhong X,Lin S,Xu H,Liang X,Wang Y,Xu J,Wang K,Guo X,Wang J,Yu M,Li C,Xie C

Thu Dec 15 00:00:00 UTC 2022

Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer's brain milieu.

Fluids and barriers of the CNS

Williams LM,Fujimoto T,Weaver RR,Logsdon AF,Evitts KM,Young JE,Banks WA,Erickson MA

Sat Feb 05 00:00:00 UTC 2022

Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer.

Nature cancer

Cappellesso F,Orban MP,Shirgaonkar N,Berardi E,Serneels J,Neveu MA,Di Molfetta D,Piccapane F,Caroppo R,Debellis L,Ostyn T,Joudiou N,Mignion L,Richiardone E,Jordan BF,Gallez B,Corbet C,Roskams T,DasGupta R,Tejpar S,Di Matteo M,Taverna D,Reshkin SJ,Topal B,Virga F,Mazzone M

Thu Dec 01 00:00:00 UTC 2022

Effect of β-hydroxybutyrate on behavioral alterations, molecular and morphological changes in CNS of multiple sclerosis mouse model.

Frontiers in aging neuroscience

Sun W,Wen M,Liu M,Wang Q,Liu Q,Li L,Siebert HC,Loers G,Zhang R,Zhang N

Thu Dec 22 00:00:00 UTC 2022

Huangqin Decoction Exerts Beneficial Effects on Rotenone-Induced Rat Model of Parkinson's Disease by Improving Mitochondrial Dysfunction and Alleviating Metabolic Abnormality of Mitochondria.

Frontiers in aging neuroscience

Gao L,Cao M,Du GH,Qin XM

Tue Aug 02 00:00:00 UTC 2022

Effects of Fibroblast Growth Factor 21 on Lactate Uptake and Usage in Mice with Diabetes-Associated Cognitive Decline.

Molecular neurobiology

Zhao L,Jiang H,Xie J,Shen D,Yi Q,Yan J,Li C,Zheng H,Gao H

Thu Sep 01 00:00:00 UTC 2022

Voglibose Regulates the Secretion of GLP-1 Accompanied by Amelioration of Ileal Inflammatory Damage and Endoplasmic Reticulum Stress in Diabetic KKAy Mice.

International journal of molecular sciences

Fu Y,Ji W,Liu Q,Zhang L,Li C,Huan Y,Lei L,Gao X,Chen L,Feng C,Lei L,Zhai J,Li P,Cao H,Liu S,Shen Z

Wed Dec 14 00:00:00 UTC 2022

Combined HP ¹³C Pyruvate and ¹³C-Glucose Fluxomic as a Potential Marker of Response to Targeted Therapies in YUMM1.7 Melanoma Xenografts.

Biomedicines

Farah C,Neveu MA,Yelek C,Bouzin C,Gallez B,Baurain JF,Mignion L,Jordan BF

Sat Mar 19 00:00:00 UTC 2022

Focal adhesion kinase-related non-kinase ameliorates liver fibrosis by inhibiting aerobic glycolysis via the FAK/Ras/c-myc/ENO1 pathway.

World journal of gastroenterology

Huang T,Li YQ,Zhou MY,Hu RH,Zou GL,Li JC,Feng S,Liu YM,Xin CQ,Zhao XK

Fri Jan 07 00:00:00 UTC 2022

Tumor Metabolism Is Affected by Obesity in Preclinical Models of Triple-Negative Breast Cancer.

Cancers

Yelek C,Mignion L,Paquot A,Bouzin C,Corbet C,Muccioli GG,Cani PD,Jordan BF

Sun Jan 23 00:00:00 UTC 2022

A Novel Role for DNA-PK in Metabolism by Regulating Glycolysis in Castration-Resistant Prostate Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Dylgjeri E,Kothari V,Shafi AA,Semenova G,Gallagher PT,Guan YF,Pang A,Goodwin JF,Irani S,McCann JJ,Mandigo AC,Chand S,McNair CM,Vasilevskaya I,Schiewer MJ,Lallas CD,McCue PA,Gomella LG,Seifert EL,Carroll JS,Butler LM,Holst J,Kelly WK,Knudsen KE

Fri Apr 01 00:00:00 UTC 2022

Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation.

Cell stem cell

Wang G,Heijs B,Kostidis S,Rietjens RGJ,Koning M,Yuan L,Tiemeier GL,Mahfouz A,Dumas SJ,Giera M,Kers J,Chuva de Sousa Lopes SM,van den Berg CW,van den Berg BM,Rabelink TJ

Thu Nov 03 00:00:00 UTC 2022

Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation.

Cell stem cell

Wang G,Heijs B,Kostidis S,Rietjens RGJ,Koning M,Yuan L,Tiemeier GL,Mahfouz A,Dumas SJ,Giera M,Kers J,Chuva de Sousa Lopes SM,van den Berg CW,van den Berg BM,Rabelink TJ

Thu Nov 03 00:00:00 UTC 2022

1,25(OH)₂D₃ alleviates LPS-induced preeclampsia-like rats impairment in the protective effect by TLR4/NF-kB pathway.

Placenta

Ma Y,Yang Y,Lv M,Zhang Y,He Q,Zhang Y,Su H,Deng X,Qian Y

Thu Dec 01 00:00:00 UTC 2022

Basigin deficiency prevents anaplerosis and ameliorates insulin resistance and hepatosteatosis.

JCI insight

Ryuge A,Kosugi T,Maeda K,Banno R,Gou Y,Zaitsu K,Ito T,Sato Y,Hirayama A,Tsubota S,Honda T,Nakajima K,Ozaki T,Kondoh K,Takahashi K,Kato N,Ishimoto T,Soga T,Nakagawa T,Koike T,Arima H,Yuzawa Y,Minokoshi Y,Maruyama S,Kadomatsu K

Fri Oct 22 00:00:00 UTC 2021

Basigin deficiency prevents anaplerosis and ameliorates insulin resistance and hepatosteatosis.

JCI insight

Fri Oct 22 00:00:00 UTC 2021

hCINAP serves a critical role in hypoxia‑induced cardiomyocyte apoptosis via modulating lactate production and mitochondrial‑mediated apoptosis signaling.

Molecular medicine reports

Xie H,Xu G,Gao Y,Yuan Z

Mon Feb 01 00:00:00 UTC 2021

Conditional Knockout of Pdha1 in Mouse Hippocampus Impairs Cognitive Function: The Possible Involvement of Lactate.

Frontiers in neuroscience

Chen W,Sun X,Zhan L,Zhou W,Bi T

Tue Nov 02 00:00:00 UTC 2021

Wogonin inhibits the growth of HT144 melanoma via regulating hedgehog signaling-mediated inflammation and glycolysis.

International immunopharmacology

Li L,Ji Y,Zhang L,Cai H,Ji Z,Gu L,Yang S

Wed Dec 01 00:00:00 UTC 2021

Immunoreactivity and a new staining method of monocarboxylate transporter 1 located in endothelial cells of cerebral vessels of human brain in distinguishing cerebral venules from arterioles.

European journal of histochemistry : EJH

Cao Y,Ao DH,Ma C,Qiu WY,Zhu YC

Fri Oct 01 00:00:00 UTC 2021

MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke.

Animal cells and systems

Yu X,Zhang R,Wei C,Gao Y,Yu Y,Wang L,Jiang J,Zhang X,Li J,Chen X

Wed Sep 20 00:00:00 UTC 2023

Ramulus Mori (Sangzhi) Alkaloids (SZ-A) Ameliorate Glucose Metabolism Accompanied by the Modulation of Gut Microbiota and Ileal Inflammatory Damage in Type 2 Diabetic KKAy Mice.

Frontiers in pharmacology

Liu Q,Liu S,Cao H,Ji W,Li C,Huan Y,Lei L,Fu Y,Gao X,Liu Y,Shen Z

Tue May 04 00:00:00 UTC 2021

Combination of Exercise and Intake of Amino Acid Mixture Synergistically Induces Beige Adipocyte Formation in Mice.

Journal of nutritional science and vitaminology

Kojima T,Esaki N,Tsuda T

Fri Sep 03 00:00:00 UTC 2021

In Vitro and In Vivo Characterization of MCT1 Inhibitor AZD3965 Confirms Preclinical Safety Compatible with Breast Cancer Treatment.

Cancers

Benyahia Z,Blackman MCNM,Hamelin L,Zampieri LX,Capeloa T,Bedin ML,Vazeille T,Schakman O,Sonveaux P

Tue Feb 02 00:00:00 UTC 2021

Effects of endurance training on the expression of host proteins involved in SARS-CoV-2 cell entry in C57BL/6J mouse.

Physiological reports

Tamura Y,Jee E,Kouzaki K,Kotani T,Nakazato K

Wed Sep 01 00:00:00 UTC 2021

Pinocembrin attenuates hemorrhagic transformation after delayed t-PA treatment in thromboembolic stroke rats by regulating endogenous metabolites.

Acta pharmacologica Sinica

Kong LL,Gao L,Wang KX,Liu NN,Liu CD,Ma GD,Yang HG,Qin XM,Du GH

Sun Aug 01 00:00:00 UTC 2021

Monocarboxylate transporter 1 promotes proliferation and invasion of renal cancer cells by mediating acetate transport.

Cell biology international

Li M,Long X,Wan H,Yin M,Yang B,Zhang F,Guo X

Tue Jun 01 00:00:00 UTC 2021

Lowering the increased intracellular pH of human-induced pluripotent stem cell-derived endothelial cells induces formation of mature Weibel-Palade bodies.

Stem cells translational medicine

Tiemeier GL,de Koning R,Wang G,Kostidis S,Rietjens RGJ,Sol WMPJ,Dumas SJ,Giera M,van den Berg CW,Eikenboom JCJ,van den Berg BM,Carmeliet P,Rabelink TJ

Wed Jul 01 00:00:00 UTC 2020

Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Journal of cellular and molecular medicine

Feng S,Zhang L,Liu X,Li G,Zhang B,Wang Z,Zhang H,Ma H

Wed Jul 01 00:00:00 UTC 2020

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Theranostics

Zhang X,Dong Y,Zhao M,Ding L,Yang X,Jing Y,Song Y,Chen S,Hu Q,Ni Y

Mon May 31 00:00:00 UTC 2021

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Theranostics

Zhang X,Dong Y,Zhao M,Ding L,Yang X,Jing Y,Song Y,Chen S,Hu Q,Ni Y

Mon May 31 00:00:00 UTC 2021

HCAR1/MCT1 Regulates Tumor Ferroptosis through the Lactate-Mediated AMPK-SCD1 Activity and Its Therapeutic Implications.

Cell reports

Zhao Y,Li M,Yao X,Fei Y,Lin Z,Li Z,Cai K,Zhao Y,Luo Z

Tue Dec 08 00:00:00 UTC 2020

Subacute ruminal acidosis suppressed the expression of MCT1 in rumen of cows.

Journal of cellular physiology