Proteintech

MCU/CCDC109A Polyclonal Antibody

MCU/CCDC109A Antibody in Western Blot (WB)

FIGURE: 1 / 5

MCU/CCDC109A Antibody (26312-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 26312-1-AP) (MCU/CCDC109A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Product Image Gallery

Product Details

26312-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MCU/CCDC109A Fusion Protein Ag24733 (155-233 aa encoded by BC034235)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DLTYHVRPP KRDLLSHENA ATLNDVKTLV QQLYTTLCIE QHQLNKEREL IERLEDLKEQ LAPLEKVRIE ISRKAEKRTT (155-233 aa encoded by BC034235)

Target Information

Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome.

Developmental cell

Geng J,Khaket TP,Pan J,Li W,Zhang Y,Ping Y,Cobos Sillero MI,Lu B

Mon Apr 10 00:00:00 UTC 2023

EFHD1, a novel mitochondrial regulator of tumor metastasis in clear cell renal cell carcinoma.

Cancer science

Meng K,Hu Y,Wang D,Li Y,Shi F,Lu J,Wang Y,Cao Y,Zhang CZ,He QY

Mon May 01 00:00:00 UTC 2023

Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes.

International journal of molecular sciences

Lan T,Zhang K,Lin F,He Q,Wu S,Xu Z,Zhang Y,Quan F

Wed Aug 03 00:00:00 UTC 2022

Ca²⁺-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak.

Cell death and differentiation

Quarato G,Llambi F,Guy CS,Min J,Actis M,Sun H,Narina S,Pruett-Miller SM,Peng J,Rankovic Z,Green DR

Fri Jul 01 00:00:00 UTC 2022

CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes.

Journal of cardiovascular translational research

Xu Y,Chen R,Yan J,Zang G,Shao C,Wang Z

Sat Oct 01 00:00:00 UTC 2022

Sulforaphane Balances Ca²⁺ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).

Molecular nutrition & food research

Tian S,Lei P,Zhang J,Sun Y,Li B,Shan Y

Thu Jul 01 00:00:00 UTC 2021

MICU3 regulates mitochondrial Ca²⁺-dependent antioxidant response in skeletal muscle aging.

Cell death & disease

Yang YF,Yang W,Liao ZY,Wu YX,Fan Z,Guo A,Yu J,Chen QN,Wu JH,Zhou J,Xiao Q

Mon Nov 29 00:00:00 UTC 2021

Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective manner.

Cell death & disease

Zhang C,Nie P,Zhou C,Hu Y,Duan S,Gu M,Jiang D,Wang Y,Deng Z,Chen J,Chen S,Wang L

Wed Feb 24 00:00:00 UTC 2021

PINK1 contained in huMSC-derived exosomes prevents cardiomyocyte mitochondrial calcium overload in sepsis via recovery of mitochondrial Ca²⁺ efflux.

Stem cell research & therapy

Zhou Q,Xie M,Zhu J,Yi Q,Tan B,Li Y,Ye L,Zhang X,Zhang Y,Tian J,Xu H

Thu May 06 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Calcium uniporter protein, mitochondrial; coiled-coil domain containing 109A; Coiled-coil domain-containing protein 109A; HsMCU

View more View less

Gene Aliases: 2010012O16Rik; AV064928; C10orf42; CCDC109A; D130073L02Rik; Gm64; MCU

View more View less

UniProt ID: (Human) Q8NE86, (Mouse) Q3UMR5

View more View less

Entrez Gene ID: (Human) 90550, (Mouse) 215999

View more View less

Function(s)

calcium channel activity protein binding uniporter activity identical protein binding

Process(es)

mitochondrial calcium ion transport calcium-mediated signaling positive regulation of insulin secretion protein complex oligomerization calcium ion transmembrane import into mitochondrion glucose homeostasis positive regulation of mitochondrial calcium ion concentration transport ion transport calcium ion transport mitochondrial calcium ion homeostasis calcium ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MCU/CCDC109A Polyclonal Antibody

MCU/CCDC109A Antibody (26312-1-AP) in WB

Product Details

26312-1-AP

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!