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Invitrogen
MDA5 Polyclonal Antibody
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MDA5 Antibody (PA5-89344) in ICC/IF
Immunofluorescence analysis of Interferon-induced helicase C domain-containing protein 1 was performed using 70% confluent log phase A549 IFN gamma, 100 units/mL for 24 hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MDA5 Polyclonal Antibody (Product # PA5-89344) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cros... View More
Product Details
PA5-89344
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human IFIH1 (NP_0714512)
Conjugate
Form
Liquid
Concentration
1.23 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal, 0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805507
Product Specific Information
Positive Samples: THP-1; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: MSNGYSTDEN FRYLISCFRA RVKMYIQVEP VLDYLTFLPA EVKEQIQRTV ATSGNMQAVE LLLSTLEKGV WHLGWTREFV EALRRTGSPL AARYMNPELT DLPSPSFENA HDEYLQLLNL LQPTLVDKLL VRDVLDKCME EELLTIEDRN RIAAAENNGN ESGVRELLKR IVQKENWFSA FLNVLRQTGN NELVQELTGS DCSES
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; Helicard; Helicase with 2 CARD domains; Interferon induced with helicase C domain protein 1; interferon induced, with helicase C domain 1; Interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5; melanoma differentiation associated protein-5; melanoma differentiation-associated gene 5; Melanoma differentiation-associated protein 5; MGC133047; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116
Gene Aliases: 9130009C22Rik; AGS7; Helicard; Hlcd; IDDM19; IFIH1; MDA-5; MDA5; RGD1560496; RH116; RLR-2; SGMRT1
UniProt ID: (Human) Q9BYX4, (Mouse) Q8R5F7
Entrez Gene ID: (Human) 64135, (Rat) 499801, (Mouse) 71586
detection of virus
response to virus
protein sumoylation
negative regulation of type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
regulation of type III interferon production
cytoplasmic pattern recognition receptor signaling pathway in response to virus
MDA-5 signaling pathway
innate immune response
regulation of apoptotic process
immune system process
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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