Invitrogen

MDA5 Recombinant Rabbit Monoclonal Antibody (33H12L34)

MDA5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

MDA5 Antibody (700360) in IHC (P)

Immunohistochemistry analysis of MDA5 showing staining in the cytoplasm of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, w... View More

MDA5 Antibody in Immunocytochemistry (ICC/IF)

MDA5 Antibody in Immunoprecipitation (IP)

MDA5 Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

MDA5 Antibody — RIG-I like receptor and IFN-lambda receptor silencing promotes HCV multicycle growth in HepG2-HFL cells. (A) Immunoblots of endogenous RIG-I, MDA5, or ß-actin were performed on lysates from HepG2-HFL cells that were transduced with lentiviral vectors expressing shRNA targeting RIG-I, MDA5, or a no targeting (NT) shRNA used as a negative control. (B) IL-29 production was assayed by ELISA 24 hpi with SeV and 24 hours transfectionwith poly(I:C). (C) HCV-GLuc multicycle growth was monitored by sampling luciferase production at 2, 4, and 7 dpi. (D) IL-29 mRNA induction was assessed in HepG2-HFL cells 30 hpi with non-reporter HCV. (E) Quantification of IFN-lambda receptor (IL28RA) mRNA levels in HepG2-HFL cells transduced with two different lentiviral vectors expressing shRNA targeting IL28RA (#1 and #2), or a control shRNA (NT). (F) HCV-GLuc multicycle growth was monitored by sampling luciferase production at 2, 4, and 7 dpi. Asterisks represent statistically significant differences between NT-shRNA expressing cells and cells expressing shRNA targeting RIG-I, MDA5, or IL-28RA at the indicated time points. {KD}

Product Details

700360

Applications

Tested Dilution

Publications

Western Blot (WB)

4-6 µg/mL

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

4-6 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

RNA Immunoprecipitation (RIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Virus

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

33H12L34

Immunogen

A recombinant protein corresponding to amino acids 883-1025 of Q9BYX4.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532316

Product Specific Information

This antibody is predicted to react with Rhesus monkey, mouse, rat, porcine and bovine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RIG-I and MDA5 are modulated by bone morphogenetic protein (BMP6) and are essential for restricting Zika virus infection in human Sertoli cells.

Frontiers in microbiology

Jiyarom B,Giannakopoulos S,Strange DP,Panova N,Gale M,Verma S

Published figure using MDA5 recombinant monoclonal antibody (Product # 700360) in Western Blot

Thu Mar 28 00:00:00 UTC 2024

Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion.

Scientific reports

Zhou X,Backman LJ,Danielson P

700360 was used in Western Blot to provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

Thu Apr 01 00:00:00 UTC 2021

Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion.

Scientific reports

Zhou X,Backman LJ,Danielson P

700360 was used in Western Blot to provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

Thu Apr 01 00:00:00 UTC 2021

Dietary Supplementation with Biobran/MGN-3 Increases Innate Resistance and Reduces the Incidence of Influenza-like Illnesses in Elderly Subjects: A Randomized, Double-Blind, Placebo-Controlled Pilot Clinical Trial.

Nutrients

Elsaid AF,Agrawal S,Agrawal A,Ghoneum M

700360 was used in Flow Cytometry to investigate the effect of Biobran/MGN-3 on ILI incidence, natural killer cell activity, and the expressions of RIG-1, and their downstream signaling genes ISG-15 and MX1.

Fri Nov 19 00:00:00 UTC 2021

Double-stranded RNA innate immune response activation from long-term adeno-associated virus vector transduction.

JCI insight

Shao W,Earley LF,Chai Z,Chen X,Sun J,He T,Deng M,Hirsch ML,Ting J,Samulski RJ,Li C

700360 was used in Western Blotting to study the role of the innate immune response at the later time points following adeno-associated viral vector transduction.

Thu Jun 21 00:00:00 UTC 2018

Rabies Virus Infection Induces the Formation of Stress Granules Closely Connected to the Viral Factories.

PLoS pathogens

Nikolic J,Civas A,Lama Z,Lagaudrière-Gesbert C,Blondel D

700360 was used in immunocytochemistry to report that rabies virus induces stress granule formation

Sat Oct 01 00:00:00 UTC 2016

HepG2 cells mount an effective antiviral interferon-lambda based innate immune response to hepatitis C virus infection.

Hepatology (Baltimore, Md.)

Israelow B,Narbus CM,Sourisseau M,Evans MJ

700360 was used in western blot to study the IFN-lambda inate immune response of HepG2 cells in response to HCV infection

Wed Oct 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; Helicard; Helicase with 2 CARD domains; Interferon induced with helicase C domain protein 1; interferon induced, with helicase C domain 1; Interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5; melanoma differentiation associated protein-5; melanoma differentiation-associated gene 5; Melanoma differentiation-associated protein 5; MGC133047; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116

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Gene Aliases: 9130009C22Rik; AGS7; Helicard; Hlcd; IDDM19; IFIH1; MDA-5; MDA5; RH116; RLR-2; SGMRT1

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UniProt ID: (Human) Q9BYX4, (Mouse) Q8R5F7

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Entrez Gene ID: (Human) 64135, (Mouse) 71586

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Function(s)

DNA binding double-stranded RNA binding single-stranded RNA binding helicase activity protein binding ATP binding zinc ion binding ribonucleoprotein complex binding nucleotide binding RNA binding hydrolase activity hydrolase activity, acting on acid anhydrides metal ion binding

Process(es)

detection of virus response to virus protein sumoylation negative regulation of type I interferon production positive regulation of interferon-alpha production positive regulation of interferon-beta production regulation of type III interferon production cytoplasmic pattern recognition receptor signaling pathway in response to virus MDA-5 signaling pathway innate immune response immune system process regulation of apoptotic process defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MDA5 Recombinant Rabbit Monoclonal Antibody (33H12L34)

MDA5 Antibody (700360) in IHC (P)

Product Details

700360

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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