Invitrogen

MDC Polyclonal Antibody, PeproTech®

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MDC Antibody (500-P107-1MG) in WB

Western Blot: To detect Human MDC (CCL22) by Western Blot analysis MDC Polyclonal Antibody (Product # 500-P107-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MDC (CCL22) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P107-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

Immunohistochemistry (IHC)

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Immunostaining (IS)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MDC (CCL22) (69 a.a.)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

AB_2929223

Product Specific Information

AA Sequence of recombinant protein: GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MDC (CCL22). Anti-Human MDC (CCL22)-specific antibody was purified by affinity chromatography employing an immobilized Human MDC (CCL22) matrix.

Sandwich ELISA: To detect Human MDC (CCL22) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MDC (CCL22) (500-P107Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MDC (CCL22).

Western Blot: To detect Human MDC (CCL22) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MDC (CCL22) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CCL22 may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Also, it is a chemotactic for monocytes. dendritic cells and natural killer cells. CCL22 is a mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulatory T Cell Apoptosis during Preeclampsia May Be Prevented by Gal-2.

International journal of molecular sciences

Meister S,Hahn L,Beyer S,Mannewitz M,Perleberg C,Schnell K,Anz D,Corradini S,Schmoeckel E,Mayr D,Hasbargen U,Zati Zehni A,Mahner S,Jeschke U,Kolben T

500-P107 was used in Immunohistochemistry to study the recruitment of regulatory T cells (Tregs) which are key players in processes mediating immune tolerance and identify galectin-2 (Gal-2) as a factor that seems to prevent the apoptosis of Tregs.

Mon Feb 07 00:00:00 UTC 2022

Cross talk between follicular Th cells and tumor cells in human follicular lymphoma promotes immune evasion in the tumor microenvironment.

Journal of immunology (Baltimore, Md. : 1950)

Rawal S,Chu F,Zhang M,Park HJ,Nattamai D,Kannan S,Sharma R,Delgado D,Chou T,Lin HY,Baladandayuthapani V,Luong A,Vega F,Fowler N,Dong C,Davis RE,Neelapu SS

Sat Jun 15 00:00:00 UTC 2013

Soluble factors from T cells inhibiting X4 strains of HIV are a mixture of β chemokines and RNases.

Proceedings of the National Academy of Sciences of the United States of America

Cocchi F,DeVico AL,Lu W,Popovic M,Latinovic O,Sajadi MM,Redfield RR,Lafferty MK,Galli M,Garzino-Demo A,Gallo RC

Tue Apr 03 00:00:00 UTC 2012

Quantification of the chemokines CCL17 and CCL22 in human colorectal adenocarcinomas.

Molecular medicine reports

Wågsäter D,Dienus O,Löfgren S,Hugander A,Dimberg J

Tue Oct 02 00:00:00 UTC 2012

Diverse and potent chemokine production by lung CD11bhigh dendritic cells in homeostasis and in allergic lung inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Beaty SR,Rose CE,Sung SS

Thu Feb 01 00:00:00 UTC 2007

Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells.

Blood

Cattoretti G,Büttner M,Shaknovich R,Kremmer E,Alobeid B,Niedobitek G

Mon May 15 00:00:00 UTC 2006

Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.

Journal of immunology (Baltimore, Md. : 1950)

Mateo T,Abu Nabah YN,Abu Taha M,Mata M,Cerdá-Nicolás M,Proudfoot AE,Stahl RA,Issekutz AC,Cortijo J,Morcillo EJ,Jose PJ,Sanz MJ

Mon May 01 00:00:00 UTC 2006

Expression of CCL28 by Reed-Sternberg cells defines a major subtype of classical Hodgkin's disease with frequent infiltration of eosinophils and/or plasma cells.

The American journal of pathology

Hanamoto H,Nakayama T,Miyazato H,Takegawa S,Hieshima K,Tatsumi Y,Kanamaru A,Yoshie O

Mon Mar 01 00:00:00 UTC 2004

Macrophage-derived chemokine (MDC/CCL22) and CCR4 are involved in the formation of T lymphocyte-dendritic cell clusters in human inflamed skin and secondary lymphoid tissue.

The American journal of pathology

Katou F,Ohtani H,Nakayama T,Ono K,Matsushima K,Saaristo A,Nagura H,Yoshie O,Motegi K

Sun Apr 01 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: AW060611; C-C motif chemokine 22; CC chemokine STCP-1; chemokine (C-C motif) ligand 22; Macrophage-derived chemokine; MDC(1-69); MGC108943; MGC34554; RP24-69A20.1; small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; stimulated T cell chemotactic protein 1; Stimulated T-cell chemotactic protein 1

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Gene Aliases: A-152E5.1; ABCD-1; CCL22; DC/B-CK; MDC; SCYA22; STCP-1

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UniProt ID: (Human) O00626

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Entrez Gene ID: (Human) 6367

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Function(s)

chemokine activity CCR chemokine receptor binding

Process(es)

monocyte chemotaxis chemotaxis inflammatory response immune response signal transduction G-protein coupled receptor signaling pathway cell-cell signaling response to virus neutrophil chemotaxis positive regulation of GTPase activity lymphocyte chemotaxis chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MDC Polyclonal Antibody, PeproTech®

MDC Antibody (500-P107-1MG) in WB

Product Details

500-P107-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Neutralization (Neu)

In vitro Assay (IV)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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