Proteintech

MDH1 Polyclonal Antibody

MDH1 Antibody in Immunocytochemistry (ICC/IF)

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MDH1 Antibody (15904-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

MDH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MDH1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15904-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MDH1 Fusion Protein Ag8744 (1-334 aa encoded by BC001484)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSEPIRVLV TGAAGQIAYS LLYSIGNGSV FGKDQPIILV LLDITPMMGV LDGVLMELQD CALPLLKDVI ATDKEDVAFK DLDVAILVGS MPRREGMERK DLLKANVKIF KSQGAALDKY AKKSVKVIVV GNPANTNCLT ASKSAPSIPK ENFSCLTRLD HNRAKAQIAL KLGVTANDVK NVIIWGNHSS TQYPDVNHAK VKLQGKEVGV YEALKDDSWL KGEFVTTVQQ RGAAVIKARK LSSAMSAAKA ICDHVRDIWF GTPEGEFVSM GVISDGNSYG VPDDLLYSFP VVIKNKTWKF VEGLPINDFS REKMDLTAKE LTEEKESAFE FLSSA (1-334 aa encoded by BC001484)

Target Information

MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Frontiers in microbiology

Li F,Fu X,Luo X,Lin Q,Liang H,Niu Y,Liu L,Li N

Tue Apr 18 00:00:00 UTC 2023

Tat-malate dehydrogenase fusion protein protects neurons from oxidative and ischemic damage by reduction of reactive oxygen species and modulation of glutathione redox system.

Scientific reports

Kwon HJ,Hahn KR,Kang MS,Choi JH,Moon SM,Yoon YS,Hwang IK,Kim DW

Thu Apr 06 00:00:00 UTC 2023

Exploration of spatial heterogeneity of tumor microenvironment in nasopharyngeal carcinoma via transcriptional digital spatial profiling.

International journal of biological sciences

Wang L,Wang D,Zeng X,Zhang Q,Wu H,Liu J,Wang Y,Liu G,Pan Y

Tue May 09 00:00:00 UTC 2023

Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells.

eLife

Hart ML,Quon E,Vigil ABG,Engstrom IA,Newsom OJ,Davidsen K,Hoellerbauer P,Carlisle SM,Sullivan LB

Wed Mar 08 00:00:00 UTC 2023

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway.

Diabetes, metabolic syndrome and obesity : targets and therapy

Mai L,He G,Chen J,Zhu J,Chen S,Yang H,Zhang M,Hou X,Ke M,Li X

Mon Jun 19 00:00:00 UTC 2023

Malonylation of Acetyl-CoA carboxylase 1 promotes hepatic steatosis and is attenuated by ketogenic diet in NAFLD.

Cell reports

Cao H,Cai Q,Guo W,Su Q,Qin H,Wang T,Xian Y,Zeng L,Cai M,Guan H,Chen S,Liang H,Xu F

Tue Apr 25 00:00:00 UTC 2023

Malonylation of Acetyl-CoA carboxylase 1 promotes hepatic steatosis and is attenuated by ketogenic diet in NAFLD.

Cell reports

Cao H,Cai Q,Guo W,Su Q,Qin H,Wang T,Xian Y,Zeng L,Cai M,Guan H,Chen S,Liang H,Xu F

Tue Apr 25 00:00:00 UTC 2023

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Journal of immunology (Baltimore, Md. : 1950)

Fan TW,Daneshmandi S,Cassel TA,Uddin MB,Sledziona J,Thompson PT,Lin P,Higashi RM,Lane AN

Tue Nov 01 00:00:00 UTC 2022

Comprehensive Analysis of Protein Acetylation and Glucose Metabolism in Mouse Brains Infected with Rabies Virus.

Journal of virology

Pei J,Yuan Y,Tian D,Huang F,Zhang C,Wang C,Zhou M,Chen H,Fu Z,Zhao L

Wed Feb 23 00:00:00 UTC 2022

iTRAQ-based quantitative proteomics analysis of the hepatoprotective effect of melatonin on ANIT-induced cholestasis in rats.

Experimental and therapeutic medicine

Wang D,Yu H,Li Y,Xu Z,Shi S,Dou D,Sun L,Zheng Z,Shi X,Deng X,Zhong X

Wed Sep 01 00:00:00 UTC 2021

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Cell research

Zhang JY,Zhou B,Sun RY,Ai YL,Cheng K,Li FN,Wang BR,Liu FJ,Jiang ZH,Wang WJ,Zhou D,Chen HZ,Wu Q

Wed Sep 01 00:00:00 UTC 2021

MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells.

Blood

Gu H,Chen C,Hao X,Su N,Huang D,Zou Y,Lin SH,Chen X,Zheng D,Liu L,Yu Z,Xie L,Zhang Y,He X,Lai X,Zhang X,Chen GQ,Zhao Y,Yang Y,Loscalzo J,Zheng J

Thu Jul 30 00:00:00 UTC 2020

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

The Journal of experimental medicine

Liu M,Wang Y,Yang C,Ruan Y,Bai C,Chu Q,Cui Y,Chen C,Ying G,Li B

Mon Mar 02 00:00:00 UTC 2020

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

iScience

Sinha T,Naash MI,Al-Ubaidi MR

Fri Apr 24 00:00:00 UTC 2020

Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility.

Journal of proteomics

Guo Y,Jiang W,Yu W,Niu X,Liu F,Zhou T,Zhang H,Li Y,Zhu H,Zhou Z,Sha J,Guo X,Chen D

Mon Sep 30 00:00:00 UTC 2019

iTRAQ-Based Proteomic Analysis Reveals Recovery of Impaired Mitochondrial Function in Ischemic Myocardium by Shenmai Formula.

Journal of proteome research

Wang Y,Zhao Y,Jiang W,Zhao X,Fan G,Zhang H,Shen P,He J,Fan X

Fri Feb 02 00:00:00 UTC 2018

iTRAQ-Based Proteomic Analysis Reveals Recovery of Impaired Mitochondrial Function in Ischemic Myocardium by Shenmai Formula.

Journal of proteome research

Wang Y,Zhao Y,Jiang W,Zhao X,Fan G,Zhang H,Shen P,He J,Fan X

Fri Feb 02 00:00:00 UTC 2018

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 UTC 2018

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Journal of ethnopharmacology

Wu L,Cao KX,Ni ZH,Li WD,Chen ZP,Cheng HB,Liu X

Fri Aug 10 00:00:00 UTC 2018

Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa.

Reproduction (Cambridge, England)

Dai J,Xu W,Zhao X,Zhang M,Zhang D,Nie D,Bao M,Wang Z,Wang L,Qiao Z

Tue Mar 01 00:00:00 UTC 2016

Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa.

Reproduction (Cambridge, England)

Dai J,Xu W,Zhao X,Zhang M,Zhang D,Nie D,Bao M,Wang Z,Wang L,Qiao Z

Tue Mar 01 00:00:00 UTC 2016

An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.

Cell

Birsoy K,Wang T,Chen WW,Freinkman E,Abu-Remaileh M,Sabatini DM

Thu Jul 30 00:00:00 UTC 2015

An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.

Cell

Birsoy K,Wang T,Chen WW,Freinkman E,Abu-Remaileh M,Sabatini DM

Thu Jul 30 00:00:00 UTC 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 UTC 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 UTC 2015

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Nature communications

Ma X,Li C,Sun L,Huang D,Li T,He X,Wu G,Yang Z,Zhong X,Song L,Gao P,Zhang H

Fri Oct 10 00:00:00 UTC 2014

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Nature communications

Ma X,Li C,Sun L,Huang D,Li T,He X,Wu G,Yang Z,Zhong X,Song L,Gao P,Zhang H

Fri Oct 10 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Aromatic alpha-keto acid reductase; Cytosolic malate dehydrogenase; diiodophenylpyruvate reductase; epididymis secretory protein Li 32; KAR; malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase, cytoplasmic; malate dehydrogenase, soluble; Malate dehydrogenase-like enzyme; soluble malate dehydrogenase

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Gene Aliases: B230377B03Rik; D17921; HEL-S-32; Mdh; MDH-s; MDH1; MDHA; Mdhl; MDL1; MGC:1375; Mor-2; MOR2

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UniProt ID: (Human) P40925, (Mouse) P14152, (Rat) O88989

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Entrez Gene ID: (Human) 4190, (Mouse) 17449, (Rat) 24551

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Function(s)

malic enzyme activity protein binding L-malate dehydrogenase activity diiodophenylpyruvate reductase activity NAD binding catalytic activity oxidoreductase activity malate dehydrogenase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Process(es)

positive regulation of defense response to virus by host carbohydrate metabolic process tricarboxylic acid cycle oxaloacetate metabolic process malate metabolic process NADH metabolic process NAD metabolic process carboxylic acid metabolic process oxidation-reduction process mitophagy in response to mitochondrial depolarization cellular carbohydrate metabolic process gluconeogenesis xenophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MDH1 Polyclonal Antibody

MDH1 Antibody (15904-1-AP) in ICC/IF

Product Details

15904-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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