Invitrogen

MDM2 Monoclonal Antibody (MD-219)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-24643

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-2 µg/mL
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MD-219

Immunogen

Recombinant mouse MDM2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794839

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Double minute 2 protein; E3 ubiquitin-protein ligase Mdm2; HGNC:6973; human homolog of; MDM2 variant FB28; MGC5370; MGC71221; Oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2

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Gene Aliases: 1700007J15Rik; AA415488; Mdm-2; Mdm2

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UniProt ID: (Mouse) P23804

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Entrez Gene ID: (Mouse) 17246

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Function(s)
p53 binding ubiquitin-protein transferase activity protein binding zinc ion binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity enzyme binding ubiquitin protein ligase binding identical protein binding peroxisome proliferator activated receptor binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding ubiquitin protein ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity scaffold protein binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein complex assembly DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest traversing start control point of mitotic cell cycle positive regulation of gene expression negative regulation of gene expression negative regulation of protein processing protein ubiquitination peptidyl-lysine modification protein catabolic process protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein localization to nucleus regulation of protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator establishment of protein localization positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of mitotic cell cycle positive regulation of protein export from nucleus negative regulation of cell cycle arrest negative regulation of signal transduction by p53 class mediator
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