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          • Primary Antibodies ›
          • MDM2 Antibodies

          NeoBiotechnologies

          MDM2 Recombinant Mouse Monoclonal Antibody (rMDM2/9214)

          View all (106) MDM2 antibodies

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          Cite MDM2 Recombinant Mouse Monoclonal Antibody (rMDM2/9214)

          • Antibody Testing Data (1)
          MDM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          MDM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

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          MDM2 Antibody (4193-MSM19-P1ABX) in IHC (P)

          Formalin-fixed, paraffin-embedded human liposarcoma stained with MDM2 Recombinant Mouse Monoclonal Antibody (rMDM2/9214). HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          MDM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Product Details

          4193-MSM19-P1ABX

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG2b, kappa

          Expression System

          CHO cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          rMDM2/9214

          Immunogen

          Recombinant human MDM2 protein fragment (exact sequence is proprietary)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A/G

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Positive Control:Human liposarcoma.

          Cellular Location: Nucleus.

          Target Information

          MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HGNC:6973; human homolog of; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2

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          Gene Aliases: ACTFS; hdm2; HDMX; MDM2

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          UniProt ID: (Human) Q00987

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          Entrez Gene ID: (Human) 4193

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          Function(s)
          p53 binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity SUMO transferase activity enzyme binding ubiquitin protein ligase binding identical protein binding peroxisome proliferator activated receptor binding ubiquitin protein ligase activity scaffold protein binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter blood vessel development blood vessel remodeling regulation of heart rate atrioventricular valve morphogenesis endocardial cushion morphogenesis ventricular septum development atrial septum development protein complex assembly DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest traversing start control point of mitotic cell cycle positive regulation of cell proliferation response to toxic substance response to carbohydrate response to iron ion positive regulation of gene expression negative regulation of protein processing viral process protein ubiquitination protein sumoylation peptidyl-lysine modification protein destabilization response to magnesium ion positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein localization to nucleus regulation of protein catabolic process response to cocaine response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to morphine negative regulation of DNA damage response, signal transduction by p53 class mediator establishment of protein localization response to ether negative regulation of transcription, DNA-templated positive regulation of mitotic cell cycle response to antibiotic positive regulation of protein export from nucleus response to steroid hormone cardiac septum morphogenesis cellular response to hydrogen peroxide negative regulation of cell cycle arrest cellular response to antibiotic cellular response to vitamin B1 cellular response to alkaloid cellular response to growth factor stimulus cellular response to peptide hormone stimulus cellular response to estrogen stimulus cellular response to hypoxia cellular response to UV-C regulation of signal transduction by p53 class mediator negative regulation of signal transduction by p53 class mediator
          It has to be done as per old AB suggested Products section.
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