Invitrogen

MED13 Polyclonal Antibody

8 Published Figures

5 References

View all (6) MED13 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

MED13 Antibody (PA5-35924) in WB

Western Blot analysis of MED13 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MED13 Polyclonal Antibody (Product # PA5-35924) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

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Product Details

PA5-35924

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MED13. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

AB_2553234

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (94%), Rat (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.

Nucleic acids research

Jackson LM,Dhoonmoon A,Hale A,Dennis KA,Schleicher EM,Nicolae CM,Moldovan GL

PA5-35924 was used in Western Blot to identify the MED12-TGFβ module as an important regulator of genomic stability and chemosensitivity in BRCA-deficient cells.

Thu Dec 16 00:00:00 UTC 2021

The Mediator complex kinase module is necessary for fructose regulation of liver glycogen levels through induction of glucose-6-phosphatase catalytic subunit (G6pc).

Molecular metabolism

Youn DY,Xiaoli AM,Zong H,Okada J,Liu L,Pessin J,Pessin JE,Yang F

PA5-35924 was used in ChIP assay to suggest that the kinase module of the Mediator complex is necessary for the transcriptional activation of metabolic genes such as G6pc and has an important role in regulating glycogen levels in the liver through altering transcription factor binding and activity at the G6pc promoter.

Tue Jun 01 00:00:00 UTC 2021

The Mediator complex kinase module is necessary for fructose regulation of liver glycogen levels through induction of glucose-6-phosphatase catalytic subunit (G6pc).

Molecular metabolism

Youn DY,Xiaoli AM,Zong H,Okada J,Liu L,Pessin J,Pessin JE,Yang F

Tue Jun 01 00:00:00 UTC 2021

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PLoS genetics

Schleicher EM,Dhoonmoon A,Jackson LM,Clements KE,Stump CL,Nicolae CM,Moldovan GL

PA5-35924 was used in Western Blotting to pave the way for personalized medicine by identifying potential biomarkers for ATR inhibitor resistance.

Sun Nov 01 00:00:00 UTC 2020

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PLoS genetics

Schleicher EM,Dhoonmoon A,Jackson LM,Clements KE,Stump CL,Nicolae CM,Moldovan GL

PA5-35924 was used in Western Blotting to pave the way for personalized medicine by identifying potential biomarkers for ATR inhibitor resistance.

Sun Nov 01 00:00:00 UTC 2020

The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity.

The Journal of biological chemistry

Youn DY,Xiaoli AM,Kwon H,Yang F,Pessin JE

PA5-35924 was used in Western Blotting to demonstrate that the assembly state of the Mediator complex undergoes physiologic regulation during normal cycles of fasting and feeding in the mouse liver.

Fri Jun 07 00:00:00 UTC 2019

The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity.

The Journal of biological chemistry

Youn DY,Xiaoli AM,Kwon H,Yang F,Pessin JE

PA5-35924 was used in Western Blotting to demonstrate that the assembly state of the Mediator complex undergoes physiologic regulation during normal cycles of fasting and feeding in the mouse liver.

Fri Jun 07 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Activator-recruited cofactor 250 kDa component; ARC250; DRIP250; Mediator complex subunit 13; Mediator of RNA polymerase II transcription subunit 13; mediator of RNA polymerase II transcription, subunit 13; mediator of RNA polymerase II transcription, subunit 13 homolog; thyroid hormone receptor associated protein 1; thyroid hormone receptor associated protein, 240 kDa subunit; Thyroid hormone receptor-associated protein 1; Thyroid hormone receptor-associated protein complex 240 kDa component; thyroid hormone receptor-associated protein complex component TRAP240; thyroid hormone receptor-associated protein, 240 kDa subunit; Trap240; Vitamin D3 receptor-interacting protein complex component DRIP250; Zmediator complex subunit 13

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Gene Aliases: 1110067M05Rik; ARC250; D030023K18; DRIP250; HSPC221; KIAA0593; MED13; THRAP1; TRAP240

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UniProt ID: (Human) Q9UHV7, (Mouse) Q5SWW4

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Entrez Gene ID: (Human) 9969, (Mouse) 327987

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Function(s)

RNA polymerase II transcription cofactor activity transcription cofactor activity transcription coactivator activity receptor activity ligand-dependent nuclear receptor transcription coactivator activity vitamin D receptor binding thyroid hormone receptor binding

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter intracellular steroid hormone receptor signaling pathway androgen receptor signaling pathway cholesterol homeostasis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter triglyceride homeostasis negative regulation of thyroid hormone receptor activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MED13 Polyclonal Antibody

MED13 Antibody (PA5-35924) in WB

Product Details

PA5-35924

Western Blot (WB)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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