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Invitrogen
MED18 Polyclonal Antibody
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FIGURE: 1 / 11
MED18 Antibody (PA5-79657) in ICC/IF
Immunocytochemistry analysis of MED18 using anti-MED18 antibody (Product # PA5-79657). MED18 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MED18 antibody (Product # PA5-79657) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize ... View More
Product Details
PA5-79657
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MED18 recombinant protein (Position: M1-M208).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746772
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human HEL whole cell, human K562 whole cell, human U251 whole cell, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue. IHC: human intestinal cancer tissue, human lung cancer tissue, mouse brain tissue, mouse small intestine tissue, rat brain tissue, rat small intestine tissue, human mammary cancer tissue. ICC/IF: A431 cell. Flow: A431 cell.
Target Information
Zinc, an essential element required for cell proliferation and differentiation, plays a role in a diverse array of cellular functions, including acting as a cofactor for numerous enzymes and transcription factors and as a neuroregulator. The zinc transporter (ZnT) family regulates the supply of zinc within cells, and its members are characterized by containing six membrane-spanning domains, a large histidine-rich intracellular loop, and a C-terminal tail. ZnT-1, a ubiquitous protein of approximately 60 kDa, localizes to the plasma membrane to aid in the export of zinc out of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ20045; mediator 18; Mediator complex subunit 18; Mediator of RNA polymerase II transcription subunit 18; mediator of RNA polymerase II transcription, subunit 18 homolog; p28b; TRAP/mediator complex subunit p28b
Gene Aliases: 2810046C01Rik; MED18; p28b
UniProt ID: (Human) Q9BUE0, (Mouse) Q9CZ82
Entrez Gene ID: (Human) 54797, (Mouse) 67219, (Rat) 682988
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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