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FIGURE: 1 / 1
MED20 Antibody (PA5-90677) in WB
Western blot analysis of MED20 in extracts of various cell lines. Samples were incubated with MED20 Polyclonal antibody (Product # PA5-90677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-90677
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_0042662)
Conjugate
Form
Liquid
Concentration
1.32 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806170
Product Specific Information
Immunogen sequence: MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADTN FDVLMVKLKG FFQSAKASKI ETRGTRYQYC DFLVKVGTVT MGPSARGISV EVEYGPCVVA SDCWSLLLEF LQSFLGSHTP GAPAVFGNRH DAVYGPADTM VQYMELFNKI RKQQQVPVAG IR; Positive Samples: DU145, Mouse brain; Cellular Location: Nucleus
Target Information
MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II, and general initiation factors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hTRFP; Mediator complex subunit 20; Mediator of RNA polymerase II transcription subunit 20; Trf (TATA binding protein-related factor)-proximal homolog; Trf (TATA binding protein-related factor)-proximal protein homolog; TRF-proximal protein homolog; ubiquitin specific protease 49
Gene Aliases: 1110011O05Rik; 2410115I17Rik; AU018348; MED20; PRO0213; TRFP; Usp49
UniProt ID: (Human) Q9H944, (Mouse) Q9R0X0, (Rat) Q5XIE9
Entrez Gene ID: (Human) 9477, (Mouse) 56771, (Rat) 316209
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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