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MED8 Antibody (PA5-79660) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of MED8 in U87 cells using MED8 Polyclonal Antibody (Product # PA5-79660). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Tubulin beta antibody overnight at 4°C. Cy3 conjugated goat anti-rabbit IgG and DyLight 488 conjugated goat anti-mouse IgG were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visuali... View More
Product Details
PA5-79660
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MED8 recombinant protein (Position: M1-R195).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746775
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human THP-1 whole cell, human HepG2 whole cell, human U251 whole cell, human Caco-2 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue. IHC: mouse testis tissue, rat testis tissue. ICC/IF: U87 cell. Flow: 293T cells.
Target Information
This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator-recruited cofactor 32 kDa component; ARC32; Mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8; mediator of RNA polymerase II transcription subunit MED8; mediator of RNA polymerase II transcription, subunit 8 homolog; MGC17544; MGC19641
Gene Aliases: 2210021A15Rik; AB041805; ARC32; MED8; MNCb-2386
UniProt ID: (Human) Q96G25, (Mouse) Q9D7W5
Entrez Gene ID: (Human) 112950, (Mouse) 80509, (Rat) 362575
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