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Invitrogen
MED9 Polyclonal Antibody
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FIGURE: 1 / 9
MED9 Antibody (PA5-79661) in ICC/IF
Immunocytochemistry analysis of MED9 using anti-MED9 antibody (Product # PA5-79661) . MED9 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MED9 antibody (Product # PA5-79661) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-79661
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MED9 recombinant protein (Position: A55-E146).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746776
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, rat heart tissue, rat kidney tissue, mouse brain tissue, mouse heart tissue, mouse kidney tissue. IHC: human colon cancer tissue, human lung cancer tissue, mouse small intestine tissue, rat small intestine tissue, human mammary cancer tissue. ICC/IF: U20S cell. Flow: MCF-7 cell.
Target Information
In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med9 (mediator complex subunit 9), also known as MED25, is a 146 amino acid nuclear protein and component of the mediator complex. Med9 directly interacts with Med4 and is encoded by a gene that maps to human chromosome 17p11.2. Chromosome 17 comprises over 2.5% of the human genome, encodes over 1,200 genes and is associated with two key tumor suppressor genes, namely, p53 and BRCA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mediator complex subunit 9; Mediator of RNA polymerase II transcription subunit 9; mediator of RNA polymerase II transcription, subunit 9 homolog; mediator subunit 25
Gene Aliases: BC019367; MED25; MED9; RGD1563669
UniProt ID: (Human) Q9NWA0, (Mouse) Q8VCS6
Entrez Gene ID: (Human) 55090, (Mouse) 192191, (Rat) 497914
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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