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MEF2D Antibody (PA5-30950) in ICC/IF
Immunofluorescence analysis of MEF2D was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MEF2D Polyclonal Antibody (Product # PA5-30950) at a dilution of 1:50 in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of ... View More
Product Details
PA5-30950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human MEF2D. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548424
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji.
Predicted reactivity: Mouse (98%), Rat (95%), Xenopus laevis (83%), Chicken (84%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686I1536; MADS box transcription enhancer factor 2, polypeptide D; MEF2D/DAZAP1 fusion; Myocyte-specific enhancer factor 2D
Gene Aliases: C80750; MEF2D
UniProt ID: (Human) Q14814, (Rat) O89038, (Mouse) Q63943
Entrez Gene ID: (Human) 4209, (Rat) 81518, (Mouse) 17261
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
activating transcription factor binding
protein homodimerization activity
histone deacetylase binding
protein heterodimerization activity
DNA binding
enzyme binding
protein dimerization activity
osteoblast differentiation
endochondral ossification
chondrocyte differentiation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
apoptotic process
nervous system development
adult heart development
skeletal muscle cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
muscle organ development
transcription, DNA-templated
multicellular organism development
cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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