Invitrogen

MEK1 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-81441

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,500-1:10,000
-

Immunocytochemistry (ICC/IF)

1:1,500-1:50,000
-

ELISA (ELISA)

1:25,000-1:50,000
-

Immunoprecipitation (IP)

1-2 µL/mg of lysate
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Mouse MEK1/MAP2K1/MKK1 Protein (Met1-Ile393)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2788650

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Mouse MEK1/MAP2K1/MKK1.

Target Information

MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; MEK2; mitogen activated protein kinase kinase 1; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen activated, kinase 1, p45

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Gene Aliases: Map2k1; MAPKK1; Mek1; MEKK1; Prkmk1

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UniProt ID: (Mouse) P31938

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Entrez Gene ID: (Mouse) 26395

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine phosphatase activity protein binding ATP binding protein C-terminus binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding protein kinase binding mitogen-activated protein kinase kinase kinase binding protein serine/threonine kinase activator activity protein N-terminus binding
Process(es)
MAPK cascade activation of MAPK activity regulation of protein phosphorylation regulation of vascular smooth muscle contraction protein phosphorylation response to oxidative stress cell cycle arrest mitotic nuclear division cell proliferation negative regulation of cell proliferation positive regulation of gene expression phosphorylation neuron differentiation keratinocyte differentiation positive regulation of cell migration melanosome transport regulation of stress-activated MAPK cascade positive regulation of transcription elongation from RNA polymerase II promoter negative regulation of homotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation positive regulation of GTPase activity positive regulation of cell differentiation positive regulation of Ras protein signal transduction vesicle transport along microtubule Golgi inheritance response to axon injury regulation of axon regeneration neuron projection morphogenesis cell motility positive regulation of axonogenesis protein heterooligomerization response to glucocorticoid lung morphogenesis trachea formation epithelial cell proliferation involved in lung morphogenesis placenta blood vessel development labyrinthine layer development ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance cellular senescence mitophagy in response to mitochondrial depolarization regulation of early endosome to late endosome transport
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