Invitrogen

MEK1 Recombinant Superclonal™ Antibody (22HCLC)

MEK1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

MEK1 Antibody (710446) in ICC/IF

Immunofluorescent analysis of MEK1 was done on 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with MEK1 Recombinant Rabbit Superclonal™ Antibody (Product # 710446) at 2 µg-4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:... View More

Product Image Gallery

MEK1 Antibody — Evaluation of the antiviral hits CDK6, MAP2K1, and MAP2K3. 293/ACE2 cells were transfected with four individual siRNAs targeting, MAP2K1 (A and B), or MAP2K3 (C and D) or three CDK6-specific siRNAs (E and F). A nontargeting scrambled siRNA was used as a control. At 48 h p.t. cells were infected with SARS-CoV-GFP at an MOI of 10 (A, C, and E) and fixed 24 h later, and GFP fluorescence (black bars) was quantified and normalized to the value measured in infected, scrambled siRNA-transfected cells (100%). The effect of siRNA transfection on cell viability was analyzed in parallel (gray bars), and values were normalized to those of scrambled siRNA-transfected control cells (100%). Each experiment was repeated at least three times (averages ± standard deviations [SD]). In parallel, siRNA-transfected cells were infected with wt SARS-CoV (MOI, 5), and at 8 h p.i., SARS-CoV N expression was monitored by Western blotting (B, D, and F). TfR was used as a loading control. Knockdown levels of the host proteins were analyzed by Western blotting (B, D, and F). The amount of SARS-CoV N protein and remaining quantity of host protein compared to that of scrambled siRNA-transfected cells (100%) is shown below each lane. All experiments were repeated at least twice. {KD}

Product Details

710446

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-4 µg

Flow Cytometry (Flow)

2-4 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

22HCLC

Immunogen

Peptide corresponding to amino acids 31-50 of human MEK1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532717

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Kinome-Wide Small Interfering RNA Screen Identifies Proviral and Antiviral Host Factors in Severe Acute Respiratory Syndrome Coronavirus Replication, Including Double-Stranded RNA-Activated Protein Kinase and Early Secretory Pathway Proteins.

Journal of virology

de Wilde AH,Wannee KF,Scholte FE,Goeman JJ,Ten Dijke P,Snijder EJ,Kikkert M,van Hemert MJ

710446 was used in western blot to identify proviral and antiviral host factors in SARS viral replication

Sat Aug 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAPK/ERK kinase 1; MAPKK 1; MEK 1; MEK2; MKK (Thr292); MKK (Thr386); MKK2; MP2K1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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Gene Aliases: CFC3; MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1

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UniProt ID: (Human) Q02750

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Entrez Gene ID: (Human) 5604

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Function(s)

protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine phosphatase activity protein binding ATP binding protein C-terminus binding protein kinase binding protein serine/threonine kinase activator activity protein N-terminus binding

Process(es)

MAPK cascade activation of MAPK activity movement of cell or subcellular component chemotaxis cell cycle arrest signal transduction heart development negative regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression peptidyl-tyrosine phosphorylation cerebellar cortex formation neuron differentiation keratinocyte differentiation thyroid gland development regulation of stress-activated MAPK cascade proteolysis in other organism positive regulation of transcription, DNA-templated thymus development regulation of axon regeneration cell motility positive regulation of axonogenesis Bergmann glial cell differentiation face development trachea formation epithelial cell proliferation involved in lung morphogenesis placenta blood vessel development labyrinthine layer development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance cellular senescence mitophagy in response to mitochondrial depolarization positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of early endosome to late endosome transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MEK1 Recombinant Superclonal™ Antibody (22HCLC)

MEK1 Antibody (710446) in ICC/IF

Product Details

710446

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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