Proteintech

MEK2 Polyclonal Antibody

MEK2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

MEK2 Antibody (11049-1-AP) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP (MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MEK2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11049-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 38 publications 38 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MEK2 Fusion Protein Ag1481 (1-400 aa encoded by BC018645)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

11049-1-AP can recognize both MEK2 and MEK1.

Immunogen sequence: MLARRKPVL PALTINPTIA EGPSPTSEGA SEANLVDLQK KLEELELDEQ QKKRLEAFLT QKAKVGELKD DDFERISELG AGNGGVVTKV QHRPSGLIMA RKLIHLEIKP AIRNQIIREL QVLHECNSPY IVGFYGAFYS DGEISICMEH MDGGSLDQVL KEAKRIPEEI LGKVSIAVLR GLAYLREKHQ IMHRDVKPSN ILVNSRGEIK LCDFGVSGQL IDSMANSFVG TRSYMAPERL QGTHYSVQSD IWSMGLSLVE LAVGRYPIPP PDAKELEAIF GRPVVDGEEG EPHSISPRPR PPGRPVSGHG MDSRPAMAIF ELLDYIVNEP PPKLPNGVFT PDFQEFVNKC LIKNPAERAD LKMLTNHTFI KRSEVEEVDF AGWLCKTLRL NQPGTPTRTA V (1-400 aa encoded by BC018645)

Target Information

MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that is known to play a critical role in mitogen growth factor signal transduction. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cytostatic Activity of Sanguinarine and a Cyanide Derivative in Human Erythroleukemia Cells Is Mediated by Suppression of c-MET/MAPK Signaling.

International journal of molecular sciences

Xu X,Deng L,Tang Y,Li J,Zhong T,Hao X,Fan Y,Mu S

Sun Apr 30 00:00:00 UTC 2023

Chitosan targets PI3K/Akt/FoxO3a axis to up-regulate FAM172A and suppress MAPK/ERK pathway to exert anti-tumor effect in osteosarcoma.

Chemico-biological interactions

Ren Z,Xiao W,He M,Bai L

Wed Mar 01 00:00:00 UTC 2023

TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway.

Scientific reports

Tang L,Guo C,Li X,Zhang B,Huang L

Mon Apr 10 00:00:00 UTC 2023

N6-Methyladenosine-Mediated Up-Regulation of FZD10 Regulates Liver Cancer Stem Cells' Properties and Lenvatinib Resistance Through WNT/β-Catenin and Hippo Signaling Pathways.

Gastroenterology

Wang J,Yu H,Dong W,Zhang C,Hu M,Ma W,Jiang X,Li H,Yang P,Xiang D

Mon May 01 00:00:00 UTC 2023

Gelatin methacryloyl (GelMA) loaded with concentrated hypoxic pretreated adipose-derived mesenchymal stem cells(ADSCs) conditioned medium promotes wound healing and vascular regeneration in aged skin.

Biomaterials research

Li S,Sun J,Yang J,Yang Y,Ding H,Yu B,Ma K,Chen M

Mon Feb 13 00:00:00 UTC 2023

BEST1 Positive Monocytes in Circulation: Visualize Intratumoral Crosstalk between Cancer Cells and Monocytes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang L,Wang Y,Yuan W,An C,Tan Q,Ma J

Thu Jun 01 00:00:00 UTC 2023

CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer.

Molecular cancer

Xing F,Gao H,Chen G,Sun L,Sun J,Qiao X,Xue J,Liu C

Tue Jan 10 00:00:00 UTC 2023

Promotion of the genipin crosslinked chitosan-fiber hydrogel loaded with sustained release of clemastine fumarate in diabetic wound repair.

International journal of biological macromolecules

Zuo R,Shi J,Jiang S,Chu M,Wang Q,Kong L,Kang Q,Guo Y,Guan J

Tue Jan 31 00:00:00 UTC 2023

HMGB3 promotes the malignant phenotypes and stemness of epithelial ovarian cancer through the MAPK/ERK signaling pathway.

Cell communication and signaling : CCS

Ma H,Qi G,Han F,Gai P,Peng J,Kong B

Fri Jun 16 00:00:00 UTC 2023

Exercise-induced endothelial Mecp2 lactylation suppresses atherosclerosis via the Ereg/MAPK signalling pathway.

Atherosclerosis

Wang Y,Chen L,Zhang M,Li X,Yang X,Huang T,Ban Y,Li Y,Li Q,Zheng Y,Sun Y,Wu J,Yu B

Thu Jun 01 00:00:00 UTC 2023

An unconventional cancer-promoting function of methamphetamine in hepatocellular carcinoma.

Life science alliance

Si Z,Yang G,Wang X,Yu Z,Pang Q,Zhang S,Qian L,Ruan Y,Huang J,Yu L

Wed Mar 01 00:00:00 UTC 2023

PLX8394, a RAF inhibitor, inhibits enterovirus 71 replication by blocking RAF/MEK/ERK signaling.

Virologica Sinica

Wu C,Zhu G,Qiu F,Ren F,Lin B,Zhang D,Yang Q,Huang C

Sat Apr 01 00:00:00 UTC 2023

ZFP281-BRCA2 prevents R-loop accumulation during DNA replication.

Nature communications

Wang Y,Ma B,Liu X,Gao G,Che Z,Fan M,Meng S,Zhao X,Sugimura R,Cao H,Zhou Z,Xie J,Lin C,Luo Z

Fri Jun 17 00:00:00 UTC 2022

ETV6 Regulates Hemin-Induced Erythroid Differentiation of K562 Cells through Mediating the Raf/MEK/ERK Pathway.

Biological & pharmaceutical bulletin

Li Z,Sun MZ,Lv X,Guo C,Liu S

Mon Apr 04 00:00:00 UTC 2022

Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.

Toxicology and applied pharmacology

Fu NJ,Xi RY,Shi XK,Li RZ,Zhang ZH,Li LY,Zhang GL,Wang F

Fri Apr 15 00:00:00 UTC 2022

Sanggenon C Suppresses Tumorigenesis of Gastric Cancer by Blocking ERK-Drp1-Mediated Mitochondrial Fission.

Journal of natural products

Chen XJ,Cui QX,Wang GL,Li XL,Zhou XL,Zhao HJ,Zhang MQ,Li MJ,He XJ,Zheng QS,Wang YL,Li D,Hong P

Fri Oct 28 00:00:00 UTC 2022

Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway.

BMC molecular and cell biology

Zhang H,Liu J,Dang Q,Wang X,Chen J,Lin X,Yang N,Du J,Shi H,Liu Y,Han J

Wed Nov 16 00:00:00 UTC 2022

Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.

ACS applied materials & interfaces

Li C,Shi S,Gao D,Li B,Song G,Chen Y,An H,Xing C

Wed Jul 20 00:00:00 UTC 2022

METTL3 Facilitates Tumor Progression by COL12A1/MAPK Signaling Pathway in Esophageal Squamous Cell Carcinoma.

Journal of Cancer

Li J,Li Z,Xu Y,Huang C,Shan B

Wed Apr 13 00:00:00 UTC 2022

Effect of propiconazole on neutrophil extracellular traps formation: Assessing the role of autophagy.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Ma Y,Li S,Ye S,Hu D,Luo S,Wei L,Xiao F

Sat Oct 01 00:00:00 UTC 2022

AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma.

Cancers

Zhang J,Ren Z,Zheng D,Song Z,Lin J,Luo Y,Zou X,Pan Y,Qi N,Li A,Liu X

Thu Sep 22 00:00:00 UTC 2022

LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.

International journal of biological sciences

Fang Y,Dou R,Huang S,Han L,Fu H,Yang C,Song J,Zheng J,Zhang X,Liu K,Xiang Z,Zhang X,Wang S,Xiong B

Thu May 12 00:00:00 UTC 2022

Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway.

Cellular & molecular biology letters

Liu P,Zu F,Chen H,Yin X,Tan X

Sat Oct 08 00:00:00 UTC 2022

AKTs do not translocate to the nucleus upon stimulation but AKT3 can constitutively signal from the nuclear envelope.

Cell reports

Wainstein E,Maik-Rachline G,Blenis J,Seger R

Tue Dec 06 00:00:00 UTC 2022

Combination of RSK inhibitor LJH-685 and FLT3 inhibitor FF-10101 promoted apoptosis and proliferation inhibition of AML cell lines.

Cellular oncology (Dordrecht, Netherlands)

Zhang S,Liu J,Lu ZY,Xue YT,Mu XR,Liu Y,Cao J,Li ZY,Li F,Xu KL,Wu QY

Sat Oct 01 00:00:00 UTC 2022

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Journal of medicinal chemistry

Zhou Z,Li Y,Ma X,Cao B,Peng T,Sheng Y,Peng H,Li R,Cao Y,Xi R,Li F,Wang M,Sun H,Zhang G,Zhang H,Hu K,Xiao W,Wang F

Thu Jun 10 00:00:00 UTC 2021

HHLA2 deficiency inhibits non-small cell lung cancer progression and THP-1 macrophage M2 polarization.

Cancer medicine

Sun W,Li S,Tang G,Sun S,Luo Y,Bai R,Han L,Jiang X,Gao Y,Huang Z,Zhang J,Gong Y,Xie C

Sun Aug 01 00:00:00 UTC 2021

miR-34a-5p functions as a tumor suppressor in head and neck squamous cell cancer progression by targeting Flotillin-2.

International journal of biological sciences

Li X,Zhao S,Fu Y,Zhang P,Zhang Z,Cheng J,Liu L,Jiang H

Mon Dec 20 00:00:00 UTC 2021

Trametinib Inhibits the Growth and Aerobic Glycolysis of Glioma Cells by Targeting the PKM2/c-Myc Axis.

Frontiers in pharmacology

Gao M,Yang J,Gong H,Lin Y,Liu J

Wed Nov 10 00:00:00 UTC 2021

Myrtenol improves brain damage and promotes angiogenesis in rats with cerebral infarction by activating the ERK1/2 signalling pathway.

Pharmaceutical biology

Huang S,Tan Z,Cai J,Wang Z,Tian Y

Wed Dec 01 00:00:00 UTC 2021

Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.

Frontiers in cell and developmental biology

Du J,Dong Z,Tan L,Tan M,Zhang F,Zhang K,Pan G,Li C,Shi S,Zhang Y,Liu Y,Cui H

Mon Aug 05 00:00:00 UTC 2024

BPTES inhibits anthrax lethal toxin-induced inflammatory response.

International immunopharmacology

Wang J,Yang D,Shen X,Wang J,Liu X,Lin J,Zhong J,Zhao Y,Qi Z

Sat Aug 01 00:00:00 UTC 2020

Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy.

Cellular signalling

Cooper LM,Hanson A,Kavanagh JA,Waddell DS

Mon Jun 01 00:00:00 UTC 2020

Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.

Clinical science (London, England : 1979)

Luo Z,Xu L,Lu J,Shen Y,Tang Y,Wang X,Wu Y,Sun H,Guo T

Fri Feb 14 00:00:00 UTC 2020

Identification of RE1-Silencing Transcription Factor as a Promoter of Metastasis in Pancreatic Cancer.

Frontiers in oncology

Jin H,Liu P,Kong L,Fei X,Gao Y,Wu T,Sun D,Tan X

Wed Sep 30 00:00:00 UTC 2020

Small Nucleolar RNA 71A Promotes Lung Cancer Cell Proliferation, Migration and Invasion via MAPK/ERK Pathway.

Journal of Cancer

Tang G,Zeng Z,Sun W,Li S,You C,Tang F,Peng S,Ma S,Luo Y,Xu J,Tian X,Zhang N,Gong Y,Xie C

Thu Oct 01 00:00:00 UTC 2020

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Cell death & disease

Li W,Cui K,Prochownik EV,Li Y

Tue May 01 00:00:00 UTC 2018

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Cell death & disease

Li W,Cui K,Prochownik EV,Li Y

Tue May 01 00:00:00 UTC 2018

SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway.

International journal of clinical and experimental pathology

Li XL,Chen XQ,Zhang MN,Chen N,Nie L,Xu M,Gong J,Shen PF,Su ZZ,Weng X,Tan JY,Zhao T,Zeng H,Zhou Q

Wed Apr 06 00:00:00 UTC 2016

SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway.

International journal of clinical and experimental pathology

Li XL,Chen XQ,Zhang MN,Chen N,Nie L,Xu M,Gong J,Shen PF,Su ZZ,Weng X,Tan JY,Zhao T,Zeng H,Zhou Q

Wed Apr 06 00:00:00 UTC 2016

hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas.

Molecular medicine reports

Wang Y,Luo H,Li Y,Chen T,Wu S,Yang L

Sun Jan 01 00:00:00 UTC 2012

hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas.

Molecular medicine reports

Wang Y,Luo H,Li Y,Chen T,Wu S,Yang L

Sun Jan 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP2K1; MAPK; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 1; MEK 2; MEK-1; MEK-2; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; OTTHUMP00000165827; PRKMK1; protein kinase, mitogen activated, kinase 2, p45

View more View less

Gene Aliases: AA589381; CFC4; MAP2K2; MAPKK2; MEK2; MK2; MKK2; PRKMK2

View more View less

UniProt ID: (Human) P36507, (Mouse) Q63932

View more View less

Entrez Gene ID: (Human) 5605, (Mouse) 26396

View more View less

Function(s)

protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity protein binding ATP binding PDZ domain binding protein complex scaffold protein serine/threonine kinase activator activity metal ion binding scaffold protein binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

MAPK cascade activation of MAPK activity regulation of protein phosphorylation protein phosphorylation phosphorylation regulation of stress-activated MAPK cascade peptidyl-serine autophosphorylation regulation of axon regeneration positive regulation of axonogenesis lung morphogenesis trachea formation epithelial cell proliferation involved in lung morphogenesis ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance positive regulation of cell motility regulation of early endosome to late endosome transport heart development negative regulation of gene expression peptidyl-tyrosine phosphorylation thyroid gland development proteolysis in other organism positive regulation of transcription, DNA-templated thymus development face development positive regulation of ERK1 and ERK2 cascade positive regulation of production of miRNAs involved in gene silencing by miRNA

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MEK2 Polyclonal Antibody

MEK2 Antibody (11049-1-AP) in ICC/IF

Product Details

11049-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!