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Invitrogen
MEK2 Polyclonal Antibody
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FIGURE: 1 / 6
MEK2 Antibody (PA5-118046) in ICC/IF
Immunofluorescence staining of MEK2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with MEK2 Polyclonal Antibody (Product # PA5-118046, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
PA5-118046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human MEK2/MAP2K2 fragment
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2902650
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that is known to play a critical role in mitogen growth factor signal transduction. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP2K1; MAPK; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MEK 1; MEK 2; MEK-1; MEK-2; mitogen-activated protein kinase kinase 2, p45; MKK1; OTTHUMP00000165827; PRKMK1
Gene Aliases: CFC4; MAP2K2; MAPKK2; MEK2; MKK2; PRKMK2
UniProt ID: (Human) P36507
Entrez Gene ID: (Human) 5605
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
PDZ domain binding
protein complex scaffold
protein serine/threonine kinase activator activity
metal ion binding
scaffold protein binding
MAPK cascade
activation of MAPK activity
heart development
negative regulation of gene expression
peptidyl-tyrosine phosphorylation
thyroid gland development
regulation of stress-activated MAPK cascade
proteolysis in other organism
peptidyl-serine autophosphorylation
positive regulation of transcription, DNA-templated
thymus development
regulation of axon regeneration
positive regulation of axonogenesis
face development
trachea formation
epithelial cell proliferation involved in lung morphogenesis
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
regulation of Golgi inheritance
positive regulation of production of miRNAs involved in gene silencing by miRNA
positive regulation of cell motility
regulation of early endosome to late endosome transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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