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MEK2 C-Term Antibody (600-401-GQ2) in IHC (P)
Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody 600-401-GN8 at 1:100 dilution for 60 minutes. Biotinylated A... View More
Product Details
600-401-GQ2
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-MEK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human MEK2 protein. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that is known to play a critical role in mitogen growth factor signal transduction. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP2K1; MAPK; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 1; MEK 2; MEK-1; MEK-2; MEK2; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; OTTHUMP00000165827; PRKMK1; protein kinase, mitogen activated, kinase 2, p45
Gene Aliases: AA589381; CFC4; MAP2K2; MAPKK2; MEK2; MK2; MKK2; PRKMK2
UniProt ID: (Human) P36507, (Rat) P36506, (Mouse) Q63932
Entrez Gene ID: (Human) 5605, (Rat) 58960, (Mouse) 26396
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
PDZ domain binding
protein complex scaffold
protein serine/threonine kinase activator activity
metal ion binding
scaffold protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
MAPK cascade
activation of MAPK activity
protein phosphorylation
peptidyl-tyrosine phosphorylation
regulation of stress-activated MAPK cascade
peptidyl-serine autophosphorylation
regulation of axon regeneration
positive regulation of axonogenesis
lung morphogenesis
trachea formation
epithelial cell proliferation involved in lung morphogenesis
ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
regulation of Golgi inheritance
positive regulation of cell motility
regulation of early endosome to late endosome transport
regulation of protein phosphorylation
phosphorylation
heart development
negative regulation of gene expression
thyroid gland development
proteolysis in other organism
positive regulation of transcription, DNA-templated
thymus development
face development
positive regulation of ERK1 and ERK2 cascade
positive regulation of production of miRNAs involved in gene silencing by miRNA
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