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Invitrogen
MEKK3 Recombinant Rabbit Monoclonal Antibody (SD0839)
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Product Details
MA5-32418
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD0839
Immunogen
Synthetic peptide within Human MEKK3 aa 1-50
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809696
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks. Northern blot analysis revealed a 4. 6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3; mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3
Gene Aliases: AW548911; MAP3K3; MAPKKK3; MEKK3; mKIAA4031
UniProt ID: (Human) Q99759, (Mouse) Q61084
Entrez Gene ID: (Human) 4215, (Mouse) 26406, (Rat) 303604
protein kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
metal ion binding
nucleotide binding
protein serine/threonine kinase activity
MAP kinase kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
molecular_function
MAPK cascade
activation of MAPKK activity
blood vessel development
intracellular signal transduction
positive regulation of I-kappaB kinase/NF-kappaB signaling
protein autophosphorylation
positive regulation of p38MAPK cascade
activation of MAPK activity
protein phosphorylation
phosphorylation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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