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Invitrogen
MELK Monoclonal Antibody (2G2)
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FIGURE: 1 / 5
MELK Antibody (MA5-17120) in ICC/IF
Immunofluorescence analysis of Maternal embryonic leucine zipper kinase was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MELK Monoclonal Antibody (2G2) (Product # MA5-17120) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary A... View More
Product Details
MA5-17120
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2G2
Immunogen
Synthesized peptide of human MELK(amino acids: 637-651:C-VYKRLVEDILSSCKV).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538591
Product Specific Information
MA5-17120 targets MELK in indirect ELISA, FACS, ICC, IHC, and IF applications and shows reactivity with Human samples.
The MA5-17120 immunogen is synthesized peptide of human MELK(amino acids: 637-651:C-VYKRLVEDILSSCKV).
MA5-17120 detects MELK which has a predicted molecular weight of approximately 74.6kDa.
Target Information
MELK is a serine/threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics. MELK has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hMELK; hPK38; Maternal embryonic leucine zipper kinase; OTTHUMP00000046113; pEg3 kinase; Protein kinase Eg3; Protein kinase PK38; RP11-8N6.1; Tyrosine-protein kinase MELK
Gene Aliases: HPK38; KIAA0175; MELK
UniProt ID: (Human) F5H689
Entrez Gene ID: (Human) 9833
G2/M transition of mitotic cell cycle
apoptotic process
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
peptidyl-tyrosine phosphorylation
hemopoiesis
intracellular signal transduction
positive regulation of apoptotic process
protein autophosphorylation
neural precursor cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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