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MEP1B Antibody (PA5-87627) in WB
Western blot analysis of MEP1B in mouse kidney. Samples were incubated with MEP1B Polyclonal antibody (Product # PA5-87627) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-87627
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 480-652 of human MEP1B (NP_0059162)
Conjugate
Form
Liquid
Concentration
0.63 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804300
Product Specific Information
Immunogen sequence: ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMSNQRSIT TDPFMTTDNG NYFWDRPSKV GTVALFSNGT QFRRGGGYGT SAFITHERLK SRDFIKGDDV YILLTVEDIS HLNSTQIQLT PAPSVQDLCS KTTCKNDGVC TVRDGKAECR CQSGEDWWYM GERCEKRGST RDT; Positive Samples: Mouse kidney; Cellular Location: Cell membrane, Single-pass type I membrane protein
Target Information
Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 3.4.24.63; Endopeptidase-2; meprin A; Meprin A subunit beta; Meprin B; meprin beta
Gene Aliases: Mep-1b; Mep1b
UniProt ID: (Mouse) Q61847
Entrez Gene ID: (Mouse) 17288
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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