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MEPCE Polyclonal Antibody

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MEPCE Antibody (11526-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer using 11526-1-AP (MEPCE antibody) at dilution of 1:50 (under 10x lens).

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Product Details

11526-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MEPCE Fusion Protein Ag2092 (369-689 aa encoded by BC018935)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SSKSEAGAR GGGQGSKEKG RGSWGGRHHH HHPLPAAGFK KQQRKFQYGN YCKYYGYRNP SCEDGRLRVL KPEWFRGRDV LDLGCNVGHL TLSIACKWGP SRMVGLDIDS RLIHSARQNI RHYLSEELRL PPQTLEGDPG AEGEEGTTTV RKRSCFPASL TASRGPIAAP QVPLDGADTS VFPNNVVFVT GNYVLDRDDL VEAQTPEYDV VLCLSLTKWV HLNWGDEGLK RMFRRIYRHL RPGGILVLEP QPWSSYGKRK TLTETIYKNY YRIQLKPEQF SSYLTSPDVG FSSYELVATP HNTSKGFQRP VYLFHKARSP SH (369-689 aa encoded by BC018935)

Target Information

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it (PubMed:17643375, PubMed:19906723, PubMed:30559425). Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed:17643375). The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PubMed:28254838). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex (PubMed:19906723). MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center (PubMed:19906723). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation.

Developmental cell

Elagib KE,Rubinstein JD,Delehanty LL,Ngoh VS,Greer PA,Li S,Lee JK,Li Z,Orkin SH,Mihaylov IS,Goldfarb AN

Mon Dec 23 00:00:00 UTC 2013

Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation.

Developmental cell

Elagib KE,Rubinstein JD,Delehanty LL,Ngoh VS,Greer PA,Li S,Lee JK,Li Z,Orkin SH,Mihaylov IS,Goldfarb AN

Mon Dec 23 00:00:00 UTC 2013

7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.

Nucleic acids research

Eilebrecht S,Brysbaert G,Wegert T,Urlaub H,Benecke BJ,Benecke A

Tue Mar 01 00:00:00 UTC 2011

7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.

Nucleic acids research

Eilebrecht S,Brysbaert G,Wegert T,Urlaub H,Benecke BJ,Benecke A

Tue Mar 01 00:00:00 UTC 2011

7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb.

RNA biology

Eilebrecht S,Benecke BJ,Benecke A

Mon Aug 27 00:00:00 UTC 2012

7SK snRNA-mediated, gene-specific cooperativity of HMGA1 and P-TEFb.

RNA biology

Eilebrecht S,Benecke BJ,Benecke A

Mon Aug 27 00:00:00 UTC 2012

7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.

Proceedings of the National Academy of Sciences of the United States of America

Barboric M,Lenasi T,Chen H,Johansen EB,Guo S,Peterlin BM

Tue May 12 00:00:00 UTC 2009

7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.

Proceedings of the National Academy of Sciences of the United States of America

Barboric M,Lenasi T,Chen H,Johansen EB,Guo S,Peterlin BM

Tue May 12 00:00:00 UTC 2009

7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.

Proceedings of the National Academy of Sciences of the United States of America

Barboric M,Lenasi T,Chen H,Johansen EB,Guo S,Peterlin BM

Tue May 12 00:00:00 UTC 2009

7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development.

Proceedings of the National Academy of Sciences of the United States of America

Barboric M,Lenasi T,Chen H,Johansen EB,Guo S,Peterlin BM

Tue May 12 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: 7SK snRNA methylphosphate capping enzyme; Bicoid-interacting protein 3 homolog; Bin3 homolog; bin3, bicoid-interacting 3, homolog; FLJ20257; MePCE

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Gene Aliases: BCDIN3; MEPCE

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UniProt ID: (Human) Q7L2J0

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Entrez Gene ID: (Human) 56257

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Function(s)

RNA methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity poly(A) RNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter RNA methylation snRNA metabolic process negative regulation of chromatin binding snRNA modification positive regulation of G1/S transition of mitotic cell cycle

Performance Guarantee

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MEPCE Polyclonal Antibody

MEPCE Antibody (11526-1-AP) in IHC (P)

Product Details

11526-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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