Invitrogen

MFN2 Recombinant Rabbit Monoclonal Antibody (7H42L13)

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MFN2 Antibody (702768) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), K562 (Lane 2), U-937 (Lane 3). Hep G2 (Lane 4), IMR-32 (Lane 5), HeLa (Lane 6) and tissue extracts of Mouse heart (Lane 7) and Mouse kidney (Lane 8).The blots were probed with Anti-MFN2 Recombinant Rabbit Monoclonal Antibody (Product # 702768, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A ~82 kDa band co... View More

Product Image Gallery

MFN2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549cells were transfected with MFN2 siRNA and decrease in signal intensity was observed in western blot application using Anti-MFN2 Monoclonal Antibody (Product # 702768). {KD}

Product Details

702768

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

7H42L13

Immunogen

Peptides corresponding to human Mitofusin 2 [1) aa391-aa410 2) aa742-aa757]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2734817

Product Specific Information

This antibody is predicted to react with Monkey, Dog, Cat, Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Autophagy Prevents Panax Notoginseng Saponins (PNS) Protection on Cardiac Myocytes Against Endoplasmic Reticulum (ER) Stress-Induced Mitochondrial Injury, Ca²⁺ Homeostasis and Associated Apoptosis.

Frontiers in pharmacology

Chen J,Li L,Bai X,Xiao L,Shangguan J,Zhang W,Zhang X,Wang S,Liu G

702768 was used in Western Blotting to investigate the roles of PNS played in TG-induced mitochondrial function, ROS accumulation and autophagy.

Fri Mar 26 00:00:00 UTC 2021

Frontiers in pharmacology

Chen J,Li L,Bai X,Xiao L,Shangguan J,Zhang W,Zhang X,Wang S,Liu G

702768 was used in Western Blotting to investigate the roles of PNS played in TG-induced mitochondrial function, ROS accumulation and autophagy.

Fri Mar 26 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: CPRP 1; HSG protein; hyperplasia suppressor; hypertension related protein 1; Hypertension-related protein 1; MFN 2; Mitochondrial assembly regulatory factor; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2

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Gene Aliases: CMT2A; CMT2A2; CPRP1; D630023P19Rik; Fzo; HMSN6A; HSG; KIAA0214; MARF; MFN2

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UniProt ID: (Human) O95140, (Mouse) Q80U63

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Entrez Gene ID: (Human) 9927, (Mouse) 170731

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Function(s)

GTPase activity protein binding GTP binding ubiquitin protein ligase binding nucleotide binding hydrolase activity GTPase binding

Process(es)

blastocyst formation protein targeting to mitochondrion autophagy apoptotic process response to unfolded protein mitochondrial membrane organization multicellular organism development mitochondrial fusion protein localization to pre-autophagosomal structure negative regulation of Ras protein signal transduction camera-type eye morphogenesis negative regulation of smooth muscle cell proliferation mitochondrion localization cell cycle arrest blood coagulation macroautophagy parkin-mediated mitophagy in response to mitochondrial depolarization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MFN2 Recombinant Rabbit Monoclonal Antibody (7H42L13)

MFN2 Antibody (702768) in WB

Product Details

702768

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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