Invitrogen

MFN2 Monoclonal Antibody (N153/5)

MFN2 Antibody in Immunocytochemistry (ICC/IF)

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MFN2 Antibody (MA5-27647) in ICC/IF

Immunofluorescent analysis of MFN2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with MFN2 monoclonal antibody (Product # MA5-27647) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Mitofusin 2 Antibody, D) Merged image. Magnifica... View More

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Product Details

MA5-27647

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

N153/5

Immunogen

Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735272

Product Specific Information

1 µg/mL of MA5-27647 was sufficient for detection of Mitofusin-2 in 20 µg of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 90kDa. No cross-reactivity against Mitofusin-1.

This antibody was formerly sold as S153-5.

Target Information

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

gp130 Activates Mitochondrial Dynamics for Hepatocyte Survival in a Model of Steatohepatitis.

Biomedicines

Shunkina D,Dakhnevich A,Shunkin E,Khaziakhmatova O,Shupletsova V,Vulf M,Komar A,Kirienkova E,Litvinova L

MA5-27647 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to investigate the effect of gp130 on the components of mitochondrial dynamics in a cellular model of steatohepatitis.

Sun Jan 29 00:00:00 UTC 2023

Tumor Necrosis Receptor Superfamily Interact with Fusion and Fission of Mitochondria of Adipose Tissue in Obese Patients without Type 2 Diabetes.

Biomedicines

Shunkina Skuratovskaia D,Komar A,Vulf M,Quang HV,Shunkin E,Kirienkova E,Dakchnevich A,Malkov D,Zatolokin P,Litvinova L

MA5-27647 was used in Western Blotting to analyze the relationship of soluble forms of receptors and ligands of the TNF superfamily in blood plasma with mitochondrial dynamics in adipose tissue (greater omentum (GO) and subcutaneous adipose tissue (Sat)) of obese patients with and without type 2 diabetes mellitus (T2DM).

Sat Sep 18 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: CPRP 1; HSG protein; hyperplasia suppressor; hypertension related protein 1; hypertension-related protein; Hypertension-related protein 1; MFN 2; Mitochondrial assembly regulatory factor; mitochondrial transmembrane GTPase FZO1A; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2

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Gene Aliases: CMT2A; CMT2A2; CPRP1; D630023P19Rik; Fzo; HMSN6A; HSG; KIAA0214; MARF; MFN2

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UniProt ID: (Human) O95140, (Mouse) Q80U63

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Entrez Gene ID: (Human) 9927, (Mouse) 170731, (Rat) 64476

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Function(s)

GTPase activity protein binding GTP binding ubiquitin protein ligase binding nucleotide binding hydrolase activity GTPase binding

Process(es)

blastocyst formation protein targeting to mitochondrion autophagy apoptotic process response to unfolded protein mitochondrial membrane organization multicellular organism development mitochondrial fusion protein localization to pre-autophagosomal structure negative regulation of Ras protein signal transduction camera-type eye morphogenesis negative regulation of smooth muscle cell proliferation mitochondrion localization cell cycle arrest blood coagulation macroautophagy parkin-mediated mitophagy in response to mitochondrial depolarization metabolic process negative regulation of cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MFN2 Monoclonal Antibody (N153/5)

MFN2 Antibody (MA5-27647) in ICC/IF

Product Details

MA5-27647

Western Blot (WB)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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