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FIGURE: 1 / 1
MGAT2 Antibody (PA5-97186) in WB
Western blot analysis of MGAT2 in extracts of various cell lines. Samples were incubated with MGAT2 Polyclonal antibody (Product # PA5-97186) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-97186
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 178-447 of human MGAT2 (NP_0023991)
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808988
Product Specific Information
Immunogen sequence: PFSIQLYPNE FPGSDPRDCP RDLPKNAALK LGCINAEYPD SFGHYREAKF SQTKHHWWWK LHFVWERVKI LRDYAGLILF LEEDHYLAPD FYHVFKKMWK LKQQECPECD VLSLGTYSAS RSFYGMADKV DVKTWKSTEH NMGLALTRNA YQKLIECTDT FCTYDDYNWD WTLQYLTVSC LPKFWKVLVP QIPRIFHAGD CGMHHKKTCR PSTQSAQIES LLNNNKQYMF PETLTISEKF TVVAISPPRK NGGWGDIRDH ELCKSYRRLQ; Positive Samples: K-562, Mouse kidney, Rat kidney; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; Beta-1,2-N-acetylglucosaminyltransferase II; beta-12-N-acetylglucosaminyltransferase II; GlcNAc-T II; GNT-II; Mannoside acetylglucosaminyltransferase 2; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II; UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II
Gene Aliases: AA407964; CDG2A; CDGS2; GLCNACTII; GNT-II; GNT2; MGAT2
UniProt ID: (Human) Q10469, (Mouse) Q921V5, (Rat) Q09326
Entrez Gene ID: (Human) 4247, (Mouse) 217664, (Rat) 94273
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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