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MGAT3 Antibody (PA5-96944) in WB
Western blot analysis of MGAT3 in mouse kidney. Samples were incubated with MGAT3 Polyclonal antibody (Product # PA5-96944) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-96944
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 364-533 of human MGAT3 (NP_0024003)
Conjugate
Form
Liquid
Concentration
1.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808746
Product Specific Information
Immunogen sequence: GCTVDMLQAV YGLDGIRLRR RQYYTMPNFR QYENRTGHIL VQWSLGSPLH FAGWHCSWCF TPEGIYFKLV SAQNGDFPRW GDYEDKRDLN YIRGLIRTGG WFDGTQQEYP PADPSEHMYA PKYLLKNYDR FHYLLDNPYQ EPRSTAAGGW RHRGPEGRPP ARGKLDEAEV; Positive Samples: SW620, U-251MG, SKOV3; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. MGAT3 (N-acetylglucosaminyltransferase III) transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Expression of this gene may be controlled by a multiple-promoter system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; GlcNAc-T III; GNT-III; mannoside acetyl glucosaminyltransferase 3; N-acetylglucosaminyltransferase III; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III; RP5-1104E15.2
Gene Aliases: 1110038J12Rik; AI848272; GGNT3; GNT-III; GNT3; MGAT3
UniProt ID: (Human) Q09327, (Rat) Q02527
Entrez Gene ID: (Human) 4248, (Mouse) 17309, (Rat) 29582
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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