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MGAT3 Antibody (MA5-55487) in WB
Western Blot analysis of various lysates using MGAT3 Recombinant Monoclonal Antibody (Product # MA5-55487) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): mouse skeletal muscle. Exposure time: 45s.
Product Details
MA5-55487
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4R7E2
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 381-480 of human MGAT3.
Conjugate
Form
Liquid
Concentration
1.6 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667727
Product Specific Information
Immunogen Sequence: LRRRQYYTMP NFRQYENRTG HILVQWSLGS PLHFAGWHCS WCFTPEGIYF KLVSAQNGDF PRWGDYEDKR DLNYIRGLIR TGGWFDGTQQ EYPPADPSEH
Target Information
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. MGAT3 (N-acetylglucosaminyltransferase III) transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Expression of this gene may be controlled by a multiple-promoter system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; glcNAc-T III; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III; RP5-1104E15.2
Gene Aliases: 1110038J12Rik; AI848272; GnT-III
Entrez Gene ID: (Mouse) 17309
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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