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Invitrogen
MGAT5 Polyclonal Antibody
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FIGURE: 1 / 2
MGAT5 Antibody (PA5-87988) in WB
Western blot analysis of MGAT5 in extracts of various cell lines. Samples were incubated with MGAT5 Polyclonal antibody (Product # PA5-87988) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-87988
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 627-741 of human GNT-V/MGAT5 (NP_0024011)
Conjugate
Form
Liquid
Concentration
0.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804566
Product Specific Information
Immunogen sequence: HGQVMWPPLS ALQVKLAEPG QSCKQVCQES QLICEPSFFQ HLNKDKDMLK YKVTCQSSEL AKDILVPSFD PKNKHCVFQG DLLLFSCAGA HPRHQRVCPC RDFIKGQVAL CKDCL; Positive Samples: LO2, 293T, Raji, Jurkat; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
Catalyzes the addition of N-acetylglucosamine in beta 1- 6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase V; beta1,6N-acetylglucosaminyltransferase V; EC 2.4.1.155; GlcNAc-T V; GNT-V; GNT-VGNT-VA; Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V
Gene Aliases: 4930471A21Rik; 5330407H02Rik; AI480971; GGNT5; GlcNAc-TV; GNT-V; GNT-VA; MGAT5
UniProt ID: (Human) Q09328, (Mouse) Q8R4G6
Entrez Gene ID: (Human) 4249, (Mouse) 107895
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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