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MGAT5B Antibody (PA5-109956) in WB
Western blot analysis of MGAT5B in mouse brain. Samples were incubated with MGAT5B Polyclonal antibody (Product # PA5-109956) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-109956
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 616-790 of human MGAT5B (NP_653278.2).
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855367
Product Specific Information
Immunogen sequence: DPYLPYEYTC EGMLERIHAY IQHQDFCRAP DPALPEAHAP QSPFVLAPNA THLEWARNTS LAPGAWPPAH ALRAWLAVPG RACTDTCLDH GLICEPSFFP FLNSQDAFLK LQVPCDSTES EMNHLYPAFA QPGQECYLQK EPLLFSCAGS NTKYRRLCPC RDFRKGQVAL CQGCL
Target Information
Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAcbeta1,2-Manalpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan. Also acts on the GlcNAcbeta1,2-Manalpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan. Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B; Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase B; beta1,6-N-acetylglucosaminyltransferase IX (GnT-IX); GlcNAc-T Vb; GNT-IX; GNT-Vb; Mannoside acetylglucosaminyltransferase 5B; N-acetylglucosaminyl-transferase Vb; N-acetylglucosaminyltranferase VB; N-acetylglucosaminyltransferase IX; UDP-N-acetylglucosamine: alpha1,6-D-mannoside beta1,6-N-acetylglucosaminyltransferase b
Gene Aliases: C330018B01; GnT-IX; Mgat5b; mGnTVB
UniProt ID: (Mouse) Q765H6
Entrez Gene ID: (Mouse) 268510
alpha-1,6-mannosyltransferase activity
alpha-1,2-mannosyltransferase activity
mannosyltransferase activity
alpha-1,3-mannosyltransferase activity
alpha-1,3-galactosyltransferase activity
UDP-glucose:glycoprotein glucosyltransferase activity
glycolipid mannosyltransferase activity
oligosaccharyl transferase activity
dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity
acetylglucosaminyltransferase activity
acetylgalactosaminyltransferase activity
galactosyltransferase activity
fucosyltransferase activity
O antigen polymerase activity
lipopolysaccharide-1,6-galactosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
cellulose synthase activity
9-phenanthrol UDP-glucuronosyltransferase activity
1-phenanthrol glycosyltransferase activity
9-phenanthrol glycosyltransferase activity
1,2-dihydroxy-phenanthrene glycosyltransferase activity
phenanthrol glycosyltransferase activity
beta-1,4-mannosyltransferase activity
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity
alpha-1,2-galactosyltransferase activity
dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity
lipopolysaccharide-1,5-galactosyltransferase activity
dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
inositol phosphoceramide synthase activity
glucosyltransferase activity
metal ion binding
alpha-(1->3)-fucosyltransferase activity
alpha-(1->6)-fucosyltransferase activity
indole-3-butyrate beta-glucosyltransferase activity
salicylic acid glucosyltransferase (ester-forming) activity
salicylic acid glucosyltransferase (glucoside-forming) activity
benzoic acid glucosyltransferase activity
chondroitin hydrolase activity
dolichyl-pyrophosphate Man7GlcNAc2 alpha-1,6-mannosyltransferase activity
cytokinin 9-beta-glucosyltransferase activity
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