Proteintech

MGMT Polyclonal Antibody

MGMT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MGMT Antibody (17195-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 17195-1-AP) (MGMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MGMT Antibody in Immunoprecipitation (IP)

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Product Details

17195-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MGMT Fusion Protein Ag9996 (1-207 aa encoded by BC000824)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDKDCEMKR TTLDSPLGKL ELSGCEQGLH EIKLLGKGTS AADAVEVPAP AAVLGGPEPL MQCTAWLNAY FHQPEAIEEF PVPALHHPVF QQESFTRQVL WKLLKVVKFG EVISYQQLAA LAGNPKAARA VGGAMRGNPV PILIPCHRVV CSSGAVGNYS GGLAVKEWLL AHEGHRLGKP GLGGSSGLAG AWLKGAGATS GSPPAGRN (1-207 aa encoded by BC000824)

Target Information

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing.

Clinical and translational medicine

Chen QW,Cai QQ,Yang Y,Dong S,Liu YY,Chen ZY,Kang CL,Qi B,Dong YW,Wu W,Zhuang LP,Shen YH,Meng ZQ,Wu XZ

Sun Jan 01 00:00:00 UTC 2023

Exosomal microRNA-222-3p increases UVB sensitivity of lens epithelium cells by suppressing MGMT.

International ophthalmology

Luo J,Li P,Kang L,Ji M,Zhou T,Qin B,Zhang J,Guan H

Mon May 01 00:00:00 UTC 2023

Exosomal microRNA-222-3p increases UVB sensitivity of lens epithelium cells by suppressing MGMT.

International ophthalmology

Luo J,Li P,Kang L,Ji M,Zhou T,Qin B,Zhang J,Guan H

Mon May 01 00:00:00 UTC 2023

GBP3 promotes glioblastoma resistance to temozolomide by enhancing DNA damage repair.

Oncogene

Xu H,Jin J,Chen Y,Wu G,Zhu H,Wang Q,Wang J,Li S,Grigore FN,Ma J,Chen CC,Lan Q,Li M

Fri Jul 01 00:00:00 UTC 2022

GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.

Oncogenesis

Zhao C,Yu H,Fan X,Niu W,Fan J,Sun S,Gong M,Zhao B,Fang Z,Chen X

Mon May 23 00:00:00 UTC 2022

A Novel Pseudogene Methylation Signature to Predict Temozolomide Outcome in Non-G-CIMP Glioblastomas.

Journal of oncology

Li B,Wang J,Liu F,Li R,Hu W,Etcheverry A,Aubry M,Mosser J,Yin A,Zhang X,Wu Y,Chen K,He Y,Wang L

Sat Jul 16 00:00:00 UTC 2022

Ubiquitination and degradation of MGMT by TRIM72 increases the sensitivity of uveal melanoma cells to Dacarbazine treatment.

Cancer biomarkers : section A of Disease markers

Li X,Yang C,Luo N,Yang Y,Guo Y,Chen P,Cun B

Thu Jun 02 00:00:00 UTC 2022

KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.

Nucleic acids research

Yang M,Wang C,Zhou M,Bao L,Wang Y,Kumar A,Xing C,Luo W,Wang Y

Fri Jun 24 00:00:00 UTC 2022

CN-3 increases TMZ sensitivity and induces ROS-dependent apoptosis and autophagy in TMZ-resistance glioblastoma.

Journal of biochemical and molecular toxicology

Xue YY,Lu YY,Sun GQ,Fang F,Ji YQ,Tang HF,Qiu PC,Cheng G

Tue Mar 01 00:00:00 UTC 2022

FoxD2-AS1 is a prognostic factor in glioma and promotes temozolomide resistance in a O⁶-methylguanine-DNA methyltransferase-dependent manner.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Shangguan W,Lv X,Tian N

Fri Nov 01 00:00:00 UTC 2019

Targeting JUN, CEBPB, and HDAC3: A Novel Strategy to Overcome Drug Resistance in Hypoxic Glioblastoma.

Frontiers in oncology

Gao Y,Liu B,Feng L,Sun B,He S,Yang Y,Wu G,E G,Liu C,Gao Y,Zhang E,Zhu B

Wed May 08 00:00:00 UTC 2024

Novel predictive epigenetic signature for temozolomide in non-G-CIMP glioblastomas.

Clinical epigenetics

Yin AA,He YL,Etcheverry A,Liu YH,Aubry M,Barnholtz-Sloan J,Liu BL,Mosser J,Lu ZF,Zhang X

Tue May 14 00:00:00 UTC 2019

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Cell research

Zheng P,Chen Q,Tian X,Qian N,Chai P,Liu B,Hu J,Blackstone C,Zhu D,Teng J,Chen J

Wed Aug 01 00:00:00 UTC 2018

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Cell research

Zheng P,Chen Q,Tian X,Qian N,Chai P,Liu B,Hu J,Blackstone C,Zhu D,Teng J,Chen J

Wed Aug 01 00:00:00 UTC 2018

Renal cell carcinoma with synchronous ipsilateral urothelial carcinoma of the renal pelvis.

Oncology letters

Lu Q,Zhuang J,Guo H

Thu Jun 01 00:00:00 UTC 2017

Knockdown of SALL4 expression using RNA interference induces cell cycle arrest, enhances early apoptosis, inhibits invasion and increases chemosensitivity to temozolomide in U251 glioma cells.

Oncology letters

Zhang L,Yan Y,Jiang Y,Qian J,Jiang L,Hu G,Lu Y,Luo C

Sun Oct 01 00:00:00 UTC 2017

Knockdown of SALL4 expression using RNA interference induces cell cycle arrest, enhances early apoptosis, inhibits invasion and increases chemosensitivity to temozolomide in U251 glioma cells.

Oncology letters

Zhang L,Yan Y,Jiang Y,Qian J,Jiang L,Hu G,Lu Y,Luo C

Sun Oct 01 00:00:00 UTC 2017

Enhancing the cytotoxicity of chemoradiation with radiation-guided delivery of anti-MGMT morpholino oligonucleotides in non-methylated solid tumors.

Cancer gene therapy

Ambady P,Wu YJ,Walker JM,Kersch C,Pagel MA,Woltjer RL,Fu R,Muldoon LL,Neuwelt EA

Tue Aug 01 00:00:00 UTC 2017

TNFR2 promotes Adriamycin resistance in breast cancer cells by repairing DNA damage.

Molecular medicine reports

Yang F,Zhao N,Wu N

Fri Sep 01 00:00:00 UTC 2017

GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment.

Journal of experimental & clinical cancer research : CR

Li J,Cai J,Zhao S,Yao K,Sun Y,Li Y,Chen L,Li R,Zhai X,Zhang J,Jiang C

Mon Nov 28 00:00:00 UTC 2016

GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment.

Journal of experimental & clinical cancer research : CR

Li J,Cai J,Zhao S,Yao K,Sun Y,Li Y,Chen L,Li R,Zhai X,Zhang J,Jiang C

Mon Nov 28 00:00:00 UTC 2016

Global and MGMT promoter hypomethylation independently associated with genomic instability of lymphocytes in subjects exposed to high-dose polycyclic aromatic hydrocarbon.

Archives of toxicology

Duan H,He Z,Ma J,Zhang B,Sheng Z,Bin P,Cheng J,Niu Y,Dong H,Lin H,Dai Y,Zhu B,Chen W,Xiao Y,Zheng Y

Fri Nov 01 00:00:00 UTC 2013

Global and MGMT promoter hypomethylation independently associated with genomic instability of lymphocytes in subjects exposed to high-dose polycyclic aromatic hydrocarbon.

Archives of toxicology

Duan H,He Z,Ma J,Zhang B,Sheng Z,Bin P,Cheng J,Niu Y,Dong H,Lin H,Dai Y,Zhu B,Chen W,Xiao Y,Zheng Y

Fri Nov 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: 6-O-methylguanine-DNA methyltransferase; methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguan; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase

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Gene Aliases: MGMT

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UniProt ID: (Human) P16455

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Entrez Gene ID: (Human) 4255

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Function(s)

DNA binding damaged DNA binding methylated-DNA-[protein]-cysteine S-methyltransferase activity calcium ion binding methyltransferase activity DNA-methyltransferase activity

Process(es)

DNA ligation DNA methylation DNA dealkylation involved in DNA repair response to toxic substance cellular response to oxidative stress response to drug negative regulation of apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process response to ethanol response to folic acid mammary gland epithelial cell differentiation cellular response to organic cyclic compound cellular response to ionizing radiation positive regulation of double-strand break repair

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MGMT Polyclonal Antibody

MGMT Antibody (17195-1-AP) in IHC (P)

Product Details

17195-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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