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Invitrogen
MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Biotin, eBioscience™
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FIGURE: 1 / 10
MHC Class I (H-2Kd) Antibody (13-5957-80) in Flow
Staining of BALB/c (left) and C57BL/6 (right) splenocytes with 0.125 µg of Mouse IgG2a K Isotype Control Biotin (Product # 13-4724-85) (blue histogram) or 0.125 µg of Anti-Mouse MHC Class I (H-2Kd) Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Product Details
13-5957-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SF1-1.1.1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_1582235
Product Specific Information
Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.
Applications Reported: This SF1-1.1.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis of Balb/c splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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