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Invitrogen
MHC Class I (RT1-A) Monoclonal Antibody (OX18), FITC, eBioscience™
FIGURE: 1 / 1
MHC Class I (RT1-A) Antibody (11-0921-82) in Flow
Staining of rat splenocytes with Anti-Rat CD3 PE (Product # 12-0030-82) and 0.5 µg of Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or 0.5 µg of Anti-Rat MHC Class I (RT1-A) FITC (right). Total viable cells were used for analysis.
Product Details
11-0921-82
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX18
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572463
Product Specific Information
Description: This OX18 monoclonal antibody recognizes a non-polymorphic determinant of rat MHC Class I, which is also known as RT1-A. Rat MHC Class I is expressed on all nucleated cells and erythrocytes.
The OX18 antibody has been reported to have functional activity.
Applications Reported: This OX18 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX18 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein is a non-polymorphic determinant of rat MHC Class I, which is also known as RT1-A. Rat MHC Class I is expressed on all nucleated cells and erythrocytes.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HA11; MHC class I RT1.Aa alpha-chain; Rat class I A; RT1 class I gene (A-1); RT1 class Ia, locus A (A1); RT1 class Ia, locus A1; RT1-A; RT1-AW2
Gene Aliases: RT1.A1(N); RT1A-1
Entrez Gene ID: (Rat) 24973, (Rat) 100188935
positive regulation of T cell mediated cytotoxicity
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
immune response
T cell tolerance induction
positive regulation of tolerance induction to nonself antigen
negative regulation of natural killer cell mediated cytotoxicity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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