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Invitrogen
MHC Class II Monoclonal Antibody (HIS19), APC, eBioscience™
FIGURE: 1 / 1
MHC Class II Antibody (17-0920-82) in Flow
PloS one 2014 -
Figure 3 Activation of HSC decreases TLR3 mediated interferon and cytokine response. (A,B,C) HSCs were isolated from control, acute CCl 4 treated rats (single injection), or chronic CCl 4 treated rats (4 weeks twice weekly injections); cells were seeded onto plates and treated with Poly(I:C) (1 ug/ml) for up to 24 hours. Interferon alpha, beta and gamma were measured and normalised to beta actin. Data are e... View More
Product Details
17-0920-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HIS19
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469424
Product Specific Information
Description: The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages.
Applications Reported: This HIS19 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: MHC 2; MHC Class II; MHC II; MHCCII
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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