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FIGURE: 1 / 1
MIB1 Antibody (PA5-97126) in WB
Western blot analysis of MIB1 in extracts of various cell lines. Samples were incubated with MIB1 Polyclonal antibody (Product # PA5-97126) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-97126
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1/DIP1 (NP_0658251)
Conjugate
Form
Liquid
Concentration
1.31 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808928
Product Specific Information
Immunogen sequence: DSEGDTPLHD AISKKRDDIL AVLLEAGADV TITNNNGFNA LHHAALRGNP SAMRVLLSKL PRPWIVDEKK DDGYTALHLA ALNNHVEVAE LLVHQGNANL DIQNVNQQTA LHLAVERQHT QIVRLLVRAG AKLDIQDKDG DTPLHEALRH HTLSQLRQLQ DMQDVGKVDA AWEPSKNTLI MGLGTQGAEK KSAASIACFL AANGADLSIR NKKGQSPLDL CPDPNLCKAL AKCHKEKVSG QVGSRSPSMI SNDSETLEEC MVCSDMKRDT LFGPCGHIAT CSLCSPRVKK CLICKEQVQS R; Positive Samples: U-87MG, A-549, HepG2, Mouse stomach, Mouse lung, Mouse kidney, Rat testis; Cellular Location: Cell membrane, Cytoplasm, centriolar satellite, centrosome, cytoskeleton, microtubule organizing center
Target Information
MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAPK-interacting protein 1; DAPK-interacting protein-1; DIP-1; E3 ubiquitin-protein ligase MIB1; Mind bomb homolog 1; mind bomb-1; mindbomb homolog 1; RING-type E3 ubiquitin transferase MIB1; skeletrophin; ubiquitin ligase mind bomb; Zinc finger ZZ type with ankyrin repeat domain protein 2
Gene Aliases: DIP-1; DIP1; E430019M12Rik; KIAA1323; LVNC7; MIB; MIB1; mKIAA1323; ZZANK2; ZZZ6
UniProt ID: (Human) Q86YT6, (Mouse) Q80SY4
Entrez Gene ID: (Human) 57534, (Mouse) 225164, (Rat) 307594
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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