Proteintech

MICAL1 Monoclonal Antibody (3F5D4)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

68297-1-IG

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000-1:50,000
-

Immunocytochemistry (ICC/IF)

1:1,000-1:4,000
-

Flow Cytometry (Flow)

0.4 µg/1x10^6 cell suspension
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

0.5-4 µg/1-3 mg of protein lysate
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3F5D4

Immunogen

Recombinant protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Immunogen sequence: HRSCFRCHTC EATLWPGGYE QHPGDGHFYC LQHLPQTDHK AEGSDRGPES PELPTPSENS MPPGLSTPTA SQEGAGPVPD PSQPTRRQIR LSSPERQRLS SLNLTPDPEM EPPPKPPRSC SALARHALES SFVGWGLPVQ SPQALVAMEK EEKESPFSSE EEEEDVPLDS DVEQALQTFA KTSGTMNNYP TWRRTLLRRA KEEEMKRFCK AQTIQRRLNE IEAALRELEA EGVKLELALR RQSSSPEQQK KLWVGQLLQL VDKKNSLVAE EAELMITVQE LNLEEKQWQL DQELRGYMNR EENLKTAADR QAEDQVLRKL VDLVNQRDAL IRFQEERRLS ELALGTGAQG

Target Information

May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [F-actin]-monooxygenase MICAL1; CasL interacting molecule MICAL; MICAL-1; microtubule associated monoxygenase, calponin and LIM domain containing 1; mMical1; Molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; protein-methionine sulfoxide oxidase MICAL1

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Gene Aliases: MICAL; MICAL-1; MICAL1; NICAL

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UniProt ID: (Human) Q8TDZ2, (Mouse) Q8VDP3, (Rat) D3ZBP4

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Entrez Gene ID: (Human) 64780, (Mouse) 171580, (Rat) 294520

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Function(s)
actin binding protein binding zinc ion binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen SH3 domain binding Rab GTPase binding FAD binding monooxygenase activity 3-(3-hydroxyphenyl)propionate hydroxylase activity oxidoreductase activity 4-chlorobenzaldehyde oxidase activity 3,5-xylenol methylhydroxylase activity phenylacetate hydroxylase activity 4-nitrophenol 4-monooxygenase activity dimethyl sulfide monooxygenase activity alpha-pinene monooxygenase [NADH] activity phenanthrene 9,10-monooxygenase activity 1-hydroxy-2-naphthoate hydroxylase activity toluene 4-monooxygenase activity xylene monooxygenase activity dibenzothiophene monooxygenase activity 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity chlorophenol 4-monooxygenase activity carbon disulfide oxygenase activity toluene 2-monooxygenase activity 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity phenanthrene 1,2-monooxygenase activity tetrahydrofuran hydroxylase activity styrene monooxygenase activity toluene-4-sulfonate monooxygenase activity toluene-sulfonate methyl-monooxygenase activity 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity 2-hydroxy-phenylacetate hydroxylase activity 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity phenanthrene 3,4-monooxygenase activity toluene 3-monooxygenase activity 4-hydroxyphenylacetate,NADH:oxygen oxidoreductase (3-hydroxylating) activity limonene monooxygenase activity 2-methylnaphthalene hydroxylase activity 1-methylnaphthalene hydroxylase activity bisphenol A hydroxylase A activity salicylate 5-hydroxylase activity isobutylamine N-hydroxylase activity branched-chain dodecylbenzene sulfonate monooxygenase activity 3-HSA hydroxylase activity 4-hydroxypyridine-3-hydroxylase activity 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase activity 6-hydroxynicotinate 3-monooxygenase activity metal ion binding tocotrienol omega-hydroxylase activity thalianol hydroxylase activity
Process(es)
negative regulation of protein phosphorylation cytoskeleton organization sulfur oxidation actin filament depolymerization negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process oxidation-reduction process signal transduction
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