Rockland

MIF Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

200-901-457

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

ELISA (ELISA)

1:1,000-1:5,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

5 mg/mL

Purification

selective precipitation

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F( c) domain of the chicken IgG molecule may interact with cells non-specifically.

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GIF; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase

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Gene Aliases: GIF; GLIF; MIF; MMIF

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UniProt ID: (Human) P14174

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Entrez Gene ID: (Human) 4282

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Function(s)
dopachrome isomerase activity receptor binding cytokine activity cytokine receptor binding protein binding chemoattractant activity phenylpyruvate tautomerase activity
Process(es)
prostaglandin biosynthetic process negative regulation of mature B cell apoptotic process inflammatory response cell surface receptor signaling pathway cell aging cell proliferation negative regulation of gene expression positive regulation of protein kinase A signaling carboxylic acid metabolic process DNA damage response, signal transduction by p53 class mediator positive regulation of B cell proliferation positive regulation of lipopolysaccharide-mediated signaling pathway negative regulation of cellular protein metabolic process negative regulation of myeloid cell apoptotic process positive regulation of peptidyl-serine phosphorylation positive regulation of phosphorylation regulation of macrophage activation negative regulation of apoptotic process positive regulation of MAP kinase activity negative regulation of DNA damage response, signal transduction by p53 class mediator innate immune response positive regulation of fibroblast proliferation positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive chemotaxis positive regulation of prostaglandin secretion involved in immune response positive regulation of myeloid leukocyte cytokine production involved in immune response protein homotrimerization positive regulation of ERK1 and ERK2 cascade negative regulation of cell cycle arrest positive regulation of arachidonic acid secretion negative regulation of cell aging negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production
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