Invitrogen

MIF Polyclonal Antibody

9 Published Figures

6 References

View all (50) MIF antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)
Published Figures (9)

MIF Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

MIF Antibody (PA5-27343) in ICC/IF

Immunofluorescent analysis of 14-3-3 zeta (green) HEK293T cells. Cells fixed with 4% formaldehyde fixed were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a 14-3-3 zeta ppolyclonal antibody (Product # PA5-27317) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature... View More

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MIF Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-27343

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:1,000-1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 1% BSA, 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544819

Product Specific Information

Recommended positive controls: Molt-4, mouse brain.

Predicted reactivity: Mouse (89%), Rat (90%), Pig (94%), Sheep (91%), Rhesus Monkey (100%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction.

Acta biochimica et biophysica Sinica

Guan D,Li Y,Cui Y,Zhao H,Dong N,Wang K,Ren D,Song T,Wang X,Jin S,Gao Y,Wang M

Published figure using MIF polyclonal antibody (Product # PA5-27343) in Western Blot

Mon Jul 10 00:00:00 UTC 2023

Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.

Life science alliance

Yamamoto Y,Kadoya K,Terkawi MA,Endo T,Konno K,Watanabe M,Ichihara S,Hara A,Kaneko K,Iwasaki N,Ishijima M

Published figure using MIF polyclonal antibody (Product # PA5-27343) in Immunohistochemistry

Sat Oct 01 00:00:00 UTC 2022

Macrophage Migration Inhibitory Factor (MIF) and Its Homologue d-Dopachrome Tautomerase (DDT) Inversely Correlate with Inflammation in Discoid Lupus Erythematosus.

Molecules (Basel, Switzerland)

Caltabiano R,De Pasquale R,Piombino E,Campo G,Nicoletti F,Cavalli E,Mangano K,Fagone P

PA5-27343 was used in Immunohistochemistry to evaluate Macrophage migration inhibitory factor in Discoid Lupus Erythematosus lesions and normal skin.

Fri Jan 01 00:00:00 UTC 2021

Macrophage Migration Inhibitory Factor (MIF) and Its Homologue d-Dopachrome Tautomerase (DDT) Inversely Correlate with Inflammation in Discoid Lupus Erythematosus.

Molecules (Basel, Switzerland)

Caltabiano R,De Pasquale R,Piombino E,Campo G,Nicoletti F,Cavalli E,Mangano K,Fagone P

PA5-27343 was used in Immunohistochemistry to evaluate Macrophage migration inhibitory factor in Discoid Lupus Erythematosus lesions and normal skin.

Fri Jan 01 00:00:00 UTC 2021

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 UTC 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 UTC 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 UTC 2020

Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1.

European journal of histochemistry : EJH

Loreto C,Caltabiano R,Graziano ACE,Castorina S,Lombardo C,Filetti V,Vitale E,Rapisarda G,Cardile V,Ledda C,Rapisarda V

PA5-27343 was used in Immunohistochemistry to demonstrate that changes in the cellular redox status brought on by cigarette smoke induce HO-1 in fibroblasts.

Thu Apr 16 00:00:00 UTC 2020

CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing.

Cellular and molecular gastroenterology and hepatology

Farr L,Ghosh S,Jiang N,Watanabe K,Parlak M,Bucala R,Moonah S

Mon May 10 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Delayed early response protein 6; DER6; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase

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Gene Aliases: GIF; GLIF; MIF; MMIF

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UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904

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Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683

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Function(s)

dopachrome isomerase activity receptor binding cytokine activity cytokine receptor binding protein binding chemoattractant activity phenylpyruvate tautomerase activity isomerase activity protein complex binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MIF Polyclonal Antibody

MIF Antibody (PA5-27343) in ICC/IF

Product Details

PA5-27343

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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