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Invitrogen
MIF Polyclonal Antibody
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MIF Antibody (PA5-81446) in ICC/IF
Immunofluorescence analysis of Macrophage migration inhibitory factor was performed using 70% confluent log phase U-87 MG cells treated with VEGF (100 ng/mL for 48hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MIF Polyclonal Antibody (Product # PA5-81446) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) High... View More
Product Details
PA5-81446
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Mouse MIF protein (Pro2-Ala115)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788655
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Mouse MIF.
Target Information
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delayed early response protein 6; DER6; GIF; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase
Gene Aliases: GIF; Glif; Mif
UniProt ID: (Mouse) P34884
Entrez Gene ID: (Mouse) 17319
prostaglandin biosynthetic process
positive regulation of protein phosphorylation
brain renin-angiotensin system
immune system process
positive regulation of acute inflammatory response
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
inflammatory response
cell surface receptor signaling pathway
spermatogenesis
cell aging
cell proliferation
negative regulation of gene expression
positive regulation of protein kinase A signaling
positive regulation of smooth muscle cell migration
carboxylic acid metabolic process
DNA damage response, signal transduction by p53 class mediator
positive regulation of B cell proliferation
positive regulation of lipopolysaccharide-mediated signaling pathway
negative regulation of cellular protein metabolic process
negative regulation of myeloid cell apoptotic process
positive regulation of peptidyl-serine phosphorylation
regulation of cell proliferation
positive regulation of phosphorylation
drinking behavior
negative regulation of apoptotic process
positive regulation of potassium ion transport
positive regulation of MAP kinase activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
innate immune response
positive regulation of glycolytic process
positive regulation of glucose import
positive regulation of fibroblast proliferation
positive regulation of axon regeneration
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of immune response
positive chemotaxis
positive regulation of prostaglandin secretion involved in immune response
positive regulation of myeloid leukocyte cytokine production involved in immune response
protein homotrimerization
positive regulation of ERK1 and ERK2 cascade
negative regulation of cell cycle arrest
positive regulation of arachidonic acid secretion
negative regulation of cell aging
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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