Invitrogen

MIF Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-94938

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MIF (98-115aa NYYDMNAANVGWNNSTFA).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806744

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Jurkat whole cell, human 293T whole cell, rat kidney tissue, rat NRK whole cell, rat PC-12 whole cell, mouse kidney tissue, mouse HBZY-1 whole cell, mouse RAW2647 whole cell. IHC: mouse brain tissue, rat brain tissue. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Delayed early response protein 6; DER6; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase

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Gene Aliases: GIF; GLIF; MIF; MMIF

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UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904

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Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683

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Function(s)
dopachrome isomerase activity receptor binding cytokine activity cytokine receptor binding protein binding chemoattractant activity phenylpyruvate tautomerase activity isomerase activity protein complex binding
Process(es)
prostaglandin biosynthetic process negative regulation of mature B cell apoptotic process inflammatory response cell surface receptor signaling pathway cell aging cell proliferation negative regulation of gene expression positive regulation of protein kinase A signaling carboxylic acid metabolic process DNA damage response, signal transduction by p53 class mediator positive regulation of B cell proliferation positive regulation of lipopolysaccharide-mediated signaling pathway negative regulation of cellular protein metabolic process negative regulation of myeloid cell apoptotic process positive regulation of peptidyl-serine phosphorylation positive regulation of phosphorylation regulation of macrophage activation negative regulation of apoptotic process positive regulation of MAP kinase activity negative regulation of DNA damage response, signal transduction by p53 class mediator innate immune response positive regulation of fibroblast proliferation positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive chemotaxis positive regulation of prostaglandin secretion involved in immune response positive regulation of myeloid leukocyte cytokine production involved in immune response protein homotrimerization positive regulation of ERK1 and ERK2 cascade negative regulation of cell cycle arrest positive regulation of arachidonic acid secretion negative regulation of cell aging negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of protein phosphorylation brain renin-angiotensin system immune system process positive regulation of acute inflammatory response positive regulation of adaptive immune response spermatogenesis positive regulation of smooth muscle cell migration regulation of cell proliferation drinking behavior positive regulation of potassium ion transport positive regulation of glycolytic process positive regulation of glucose import positive regulation of axon regeneration positive regulation of immune response hair follicle development brain development aging metabolic process response to heat response to mechanical stimulus response to hormone response to inorganic substance response to estradiol response to lipopolysaccharide response to progesterone response to menaquinone response to insulin response to vitamin E wound healing response to drug skin development response to glucocorticoid cellular response to hydrogen peroxide cellular response to hypoxia
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