Invitrogen

MINA53 Monoclonal Antibody (M532)

MINA53 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

MINA53 Antibody (39-7300) in ICC/IF

Immunofluorescence analysis of MINA53 was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MINA53 (M532) Mouse Monoclonal Antibody (Product # 39-7300) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

MINA53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

39-7300

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

ELISA (ELISA)

Assay-dependent

ChIP assay (ChIP)

View 4 publications 4 publications

Competition Assay (CA)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

M532

Immunogen

Recombinant full-length human Mina53

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533432

Target Information

MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

39-7300 was used in Western Blot to suggest that mdig is a key mediator for the severity of COVID-19 in response to environmental exposure and targeting mdig may be the one of the effective strategies in ameliorating the symptom and reducing the mortality of COVID-19.

Fri Aug 06 00:00:00 UTC 2021

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

Fri Aug 06 00:00:00 UTC 2021

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

Fri Aug 06 00:00:00 UTC 2021

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

Fri Aug 06 00:00:00 UTC 2021

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Cancer research

Zhang L,Huo Q,Ge C,Zhao F,Zhou Q,Chen X,Tian H,Chen T,Xie H,Cui Y,Yao M,Li H,Li J

39-7300 was used in Western Blot to describe the mechanism by which zinc finger protein 143 promotes hepatocellular carcinoma proliferation and provide important clues for exploring new targets and strategies for clinical treatment of human liver cancer.

Mon Jun 15 00:00:00 UTC 2020

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Cancer research

Zhang L,Huo Q,Ge C,Zhao F,Zhou Q,Chen X,Tian H,Chen T,Xie H,Cui Y,Yao M,Li H,Li J

Mon Jun 15 00:00:00 UTC 2020

CRISPR-Cas9 gene editing causes alternative splicing of the targeting mRNA.

Biochemical and biophysical research communications

Zhang Q,Fu Y,Thakur C,Bi Z,Wadgaonkar P,Qiu Y,Xu L,Rice M,Zhang W,Almutairy B,Chen F

39-7300 was used in Western Blot to conclude that cautions should be taken when using CRISPR-Cas9 strategy to edit human genes due to the unintended alterative splicing of the target mRNAs.

Sun Jul 12 00:00:00 UTC 2020

MINA53 deficiency leads to glioblastoma cell apoptosis via inducing DNA replication stress and diminishing DNA damage response.

Cell death & disease

Xuan F,Huang M,Zhao E,Cui H

39-7300 was used in Chromatin immunoprecipitation to determine the role of MYC-induced nuclear antigen in DNA replication initiation in glioblastoma cells.

Wed Oct 17 00:00:00 UTC 2018

MINA53 deficiency leads to glioblastoma cell apoptosis via inducing DNA replication stress and diminishing DNA damage response.

Cell death & disease

Xuan F,Huang M,Zhao E,Cui H

39-7300 was used in Chromatin immunoprecipitation to determine the role of MYC-induced nuclear antigen in DNA replication initiation in glioblastoma cells.

Wed Oct 17 00:00:00 UTC 2018

Transcriptional activation of Mina by Sp1/3 factors.

PloS one

Lian S,Potula HH,Pillai MR,Van Stry M,Koyanagi M,Chung L,Watanabe M,Bix M

39-7300 was used in western blot to characterize the Mina promoter.

Mon Sep 08 00:00:00 UTC 2014

Mina, an Il4 repressor, controls T helper type 2 bias.

Nature immunology

Okamoto M,Van Stry M,Chung L,Koyanagi M,Sun X,Suzuki Y,Ohara O,Kitamura H,Hijikata A,Kubo M,Bix M

39-7300 was used in ChIP assay and western blot to identify Mina as a genetic determinant of T helper type 2 bias

Sat Aug 01 00:00:00 UTC 2009

Increased expression of a Myc target gene Mina53 in human colon cancer.

The American journal of pathology

Teye K,Tsuneoka M,Arima N,Koda Y,Nakamura Y,Ueta Y,Shirouzu K,Kimura H

Thu Jan 01 00:00:00 UTC 2004

Increased expression of a Myc target gene Mina53 in human colon cancer.

The American journal of pathology

Teye K,Tsuneoka M,Arima N,Koda Y,Nakamura Y,Ueta Y,Shirouzu K,Kimura H

Thu Jan 01 00:00:00 UTC 2004

Increased expression of a Myc target gene Mina53 in human colon cancer.

The American journal of pathology

Teye K,Tsuneoka M,Arima N,Koda Y,Nakamura Y,Ueta Y,Shirouzu K,Kimura H

Thu Jan 01 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: 60S ribosomal protein L27a histidine hydroxylase; Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA; DKFZp762O1912; FLJ14393; Histone lysine demethylase MINA; MINA; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; Nucleolar protein 52; Protein mina53; Ribosomal oxygenase 2; Ribosomal oxygenase MINA; ROX

View more View less

Gene Aliases: MDIG; MINA; MINA53; NO52; RIOX2; ROX

View more View less

UniProt ID: (Human) Q8IUF8

View more View less

Entrez Gene ID: (Human) 84864

View more View less

Function(s)

transcriptional repressor activity, RNA polymerase II transcription factor binding iron ion binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated peptidyl-amino acid modification ribosome biogenesis post-translational protein modification negative regulation of transcription, DNA-templated oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MINA53 Monoclonal Antibody (M532)

MINA53 Antibody (39-7300) in ICC/IF

Product Details

39-7300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Competition Assay (CA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!