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MIP-1 beta Antibody (214-401-B85) in WB
Western blot using Rocklands protein-A purified Anti-MIP-1ß (CCL4) antibody shows detection of recombinant swine MIP-1ß (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20,000 for 45 min at room temperature.
Product Details
214-401-B85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1beta protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 90% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.
Target Information
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 4; Macrophage inflammatory protein 1-beta; MGC104418; MGC126025; MGC126026; MIP-1-beta; MIP1-beta; MIP1beta; RP23-320E6.8; Small-inducible cytokine A4
Gene Aliases: CCL4; MIP-1BETA; MIP1B; SCY4A
UniProt ID: (Pig) Q711P4
Entrez Gene ID: (Pig) 396668
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