Proteintech

MITF Polyclonal Antibody

FIGURE: 1 / 6

MITF Antibody (13092-1-AP) in WB

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MITF Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13092-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 20 publications 20 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1:50

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MITF Fusion Protein Ag3679 (1-91 aa encoded by BC012503)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLEMLEYNH YQVQTHLENP TKYHIQQAQR QQVKQYLSTT LANKHANQVL SLPCPNQPGD HVMPPVPGSS APNSPMAMLT LNSNCEKEFM KQ (1-91 aa encoded by BC012503)

Target Information

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Silver Carp (Hypophthalmichthys molitrix) Scale Collagen Peptides-1 (SCPs1) Inhibit Melanogenesis through Downregulation of the cAMP-CREB Signaling Pathway.

Nutrients

Li HL,Li MJ,Xiong GQ,Cai J,Liao T,Zu XY

Wed May 24 00:00:00 UTC 2023

MiR-182 Is Upregulated in Prostate Cancer and Contributes to Tumor Progression by Targeting MITF.

International journal of molecular sciences

Stafford MYC,McKenna DJ

Tue Jan 17 00:00:00 UTC 2023

Silk Fibroin-Based Hydrogel Microneedles Deliver α-MSH to Promote Melanosome Delivery for Vitiligo Treatment.

ACS biomaterials science & engineering

Chong S,Wei C,Feng L,Guo R

Mon Jun 12 00:00:00 UTC 2023

Effects of MITF on marker protein expression of multivesicular bodies and miRNA omics of extracellular vesicles of mice melanocyte cell line.

Acta histochemica

Zhao L,Han H,Li Y,Pang Q

Wed Feb 01 00:00:00 UTC 2023

The human vault RNA enhances tumorigenesis and chemoresistance through the lysosome in hepatocellular carcinoma.

Autophagy

Ferro I,Gavini J,Gallo S,Bracher L,Landolfo M,Candinas D,Stroka DM,Polacek N

Sat Jan 01 00:00:00 UTC 2022

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.

iScience

Tian H,Chen Z,Zhu X,Ou Q,Wang Z,Wu B,Xu JY,Jin C,Gao F,Wang J,Zhang J,Zhang J,Lu L,Xu GT

Fri Oct 21 00:00:00 UTC 2022

The role of KLF4 in melanogenesis and homeostasis in sheep melanocytes.

Acta histochemica

Qin H,Chen Y,Wang S,Ge S,Pang Q

Sat Jan 01 00:00:00 UTC 2022

1,4-dihydroxy quininib modulates the secretome of uveal melanoma tumour explants and a marker of oxidative phosphorylation in a metastatic xenograft model.

Frontiers in medicine

Slater K,Bosch R,Smith KF,Jahangir CA,Garcia-Mulero S,Rahman A,O'Connell F,Piulats JM,O'Neill V,Horgan N,Coupland SE,O'Sullivan J,Gallagher WM,Villanueva A,Kennedy BN

Thu Feb 02 00:00:00 UTC 2023

Generation and Differentiation of Induced Pluripotent Stem Cells from Mononuclear Cells in An Age-Related Macular Degeneration Patient.

Cell journal

Wang T,Liu J,Chen J,Qin B

Thu Dec 01 00:00:00 UTC 2022

Extracellular Vesicles from Korean Codium fragile and Sargassum fusiforme Negatively Regulate Melanin Synthesis.

Molecules and cells

Jang B,Chung H,Jung H,Song HK,Park E,Choi HS,Jung K,Choe H,Yang S,Oh ES

Sun Oct 31 00:00:00 UTC 2021

TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.

Theranostics

Zhou K,Zheng Z,Li Y,Han W,Zhang J,Mao Y,Chen H,Zhang W,Liu M,Xie L,Zhang H,Xu H,Xiao J

Fri May 28 00:00:00 UTC 2021

Ultraviolet B irradiation enhances the secretion of exosomes by human primary melanocytes and changes their exosomal miRNA profile.

PloS one

Shen Z,Sun J,Shao J,Xu J

Mon Oct 26 00:00:00 UTC 2020

D-tyrosine adds an anti-melanogenic effect to cosmetic peptides.

Scientific reports

Park J,Jung H,Jang B,Song HK,Han IO,Oh ES

Tue Jan 14 00:00:00 UTC 2020

Glucose Exerts an Anti-Melanogenic Effect by Indirect Inactivation of Tyrosinase in Melanocytes and a Human Skin Equivalent.

International journal of molecular sciences

Lee SH,Bae IH,Lee ES,Kim HJ,Lee J,Lee CS

Tue Mar 03 00:00:00 UTC 2020

mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulation.

The Journal of clinical investigation

Asrani K,Murali S,Lam B,Na CH,Phatak P,Sood A,Kaur H,Khan Z,Noë M,Anchoori RK,Talbot CC,Smith B,Skaro M,Lotan TL

Mon Dec 02 00:00:00 UTC 2019

D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity.

Pigment cell & melanoma research

Park J,Jung H,Kim K,Lim KM,Kim JY,Jho EH,Oh ES

Tue May 01 00:00:00 UTC 2018

D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity.

Pigment cell & melanoma research

Park J,Jung H,Kim K,Lim KM,Kim JY,Jho EH,Oh ES

Tue May 01 00:00:00 UTC 2018

Coumestrol Down-Regulates Melanin Production in Melan-a Murine Melanocytes through Degradation of Tyrosinase.

Biological & pharmaceutical bulletin

Hwang JA,Park NH,Na YJ,Lee HK,Lee JH,Kim YJ,Lee CS

Mon Jul 24 00:00:00 UTC 2017

Coumestrol Down-Regulates Melanin Production in Melan-a Murine Melanocytes through Degradation of Tyrosinase.

Biological & pharmaceutical bulletin

Hwang JA,Park NH,Na YJ,Lee HK,Lee JH,Kim YJ,Lee CS

Mon Jul 24 00:00:00 UTC 2017

Phenylacylphenol derivatives with anti-melanogenic activity from Stewartia pseudocamellia.

Archives of pharmacal research

Seong ZK,Kim HS,Won YM,Kim JL,Song HH,Kim DY,Oh SR,Cho HW,Cho JH,Lee HK

Sun May 01 00:00:00 UTC 2016

Triterpene glycosides with stimulatory activity on melanogenesis from the aerial parts of Weigela subsessilis.

Archives of pharmacal research

Won YM,Seong ZK,Kim JL,Kim HS,Song HH,Kim DY,Kim JH,Oh SR,Cho HW,Cho JH,Lee HK

Sat Aug 01 00:00:00 UTC 2015

Triterpene glycosides with stimulatory activity on melanogenesis from the aerial parts of Weigela subsessilis.

Archives of pharmacal research

Won YM,Seong ZK,Kim JL,Kim HS,Song HH,Kim DY,Kim JH,Oh SR,Cho HW,Cho JH,Lee HK

Sat Aug 01 00:00:00 UTC 2015

(E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol Enhances Melanogenesis through Increasing Upstream Stimulating Factor-1-Mediated Tyrosinase Expression.

PloS one

Park J,Chung H,Bang SH,Han AR,Seo EK,Chang SE,Kang DH,Oh ES

Mon Jun 27 00:00:00 UTC 2016

(E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol Enhances Melanogenesis through Increasing Upstream Stimulating Factor-1-Mediated Tyrosinase Expression.

PloS one

Park J,Chung H,Bang SH,Han AR,Seo EK,Chang SE,Kang DH,Oh ES

Mon Jun 27 00:00:00 UTC 2016

Liver X receptor activation inhibits melanogenesis through the acceleration of ERK-mediated MITF degradation.

The Journal of investigative dermatology

Lee CS,Park M,Han J,Lee JH,Bae IH,Choi H,Son ED,Park YH,Lim KM

Mon Apr 01 00:00:00 UTC 2013

Liver X receptor activation inhibits melanogenesis through the acceleration of ERK-mediated MITF degradation.

The Journal of investigative dermatology

Lee CS,Park M,Han J,Lee JH,Bae IH,Choi H,Son ED,Park YH,Lim KM

Mon Apr 01 00:00:00 UTC 2013

Liver X receptor activation inhibits melanogenesis through the acceleration of ERK-mediated MITF degradation.

The Journal of investigative dermatology

Lee CS,Park M,Han J,Lee JH,Bae IH,Choi H,Son ED,Park YH,Lim KM

Mon Apr 01 00:00:00 UTC 2013

Identification of sake extract as a new anti-melanogenic ingredient by in vitro and clinical trials.

Natural product communications

Hwang JA,Goh MJ,Kim EJ,Lee MR,Park NH,Na YJ,Cho JC,Lee HK

Fri Nov 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: bHLHe32; black eyed white; Class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor; microphthalmia; Microphthalmia-associated transcription factor

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Gene Aliases: BCC2; BHLHE32; Bw; CMM8; Gsfbcc2; MI; MITF; Vit; Vitiligo; Wh; WS2; WS2A

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UniProt ID: (Human) O75030, (Rat) O88368, (Mouse) Q08874

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Entrez Gene ID: (Human) 4286, (Rat) 25094, (Mouse) 17342

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding protein dimerization activity RNA polymerase II core promoter sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly multicellular organism development positive regulation of gene expression protein sumoylation melanocyte differentiation regulation of apoptotic process regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter mast cell migration positive regulation of DNA-templated transcription, initiation regulation of RNA biosynthetic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of gene expression Wnt signaling pathway cell differentiation osteoclast differentiation regulation of cell proliferation camera-type eye development negative regulation of apoptotic process pigmentation canonical Wnt signaling pathway involved in negative regulation of apoptotic process cell fate commitment bone remodeling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MITF Polyclonal Antibody

MITF Antibody (13092-1-AP) in WB

Product Details

13092-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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