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MLF1 Antibody (600-401-A92) in IHC (P)
Immunohistochemistry of rabbit anti-MLF1 antibody. Tissue: brain, cerebellum. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-MLF1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Product Details
600-401-A92
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a 200 residue recombinant protein corresponding to the amino terminal end of human MLF1IP protein.
Conjugate
Form
Liquid
Concentration
0.88 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human MLF1IP protein. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, chimpanzee (98%), horse (72%), bovine (69%), dog (65%), mouse (56%), rat (55%) and chicken (47%) sources based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
SERBP1 may play a role in the regulation of mRNA stability. It binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MLF-1; Myelodysplasia-myeloid leukemia factor 1; Myeloid leukemia factor 1; testis tissue sperm-binding protein Li 49e
Gene Aliases: MLF1
UniProt ID: (Human) P58340
Entrez Gene ID: (Human) 4291
Performance Guarantee
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