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MLH1 Antibody (CF810258) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLH1 (RC201607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MLH1. (1. Positive lysates LY400096 (100 µg) and LC400096 (20 µg) can be purchased separately from OriGene.
Product Details
CF810258
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI5F3
Immunogen
Human recombinant protein fragment corresponding to amino acids 299-527 of human MLH1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; MGC5172; mismatch repair protein 1; MLH-1; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1
Gene Aliases: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MLH1
UniProt ID: (Human) P40692, (Rat) P97679, (Mouse) Q9JK91
Entrez Gene ID: (Human) 4292, (Rat) 81685, (Mouse) 17350
nuclear-transcribed mRNA poly(A) tail shortening
resolution of meiotic recombination intermediates
response to hypoxia
somatic recombination of immunoglobulin genes involved in immune response
DNA repair
mismatch repair
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
male meiosis chromosome segregation
meiotic nuclear division
synapsis
reciprocal meiotic recombination
male meiosis
spermatogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
response to toxic substance
female meiosis chromosome segregation
somatic hypermutation of immunoglobulin genes
somatic recombination of immunoglobulin gene segments
response to drug
meiotic metaphase I plate congression
meiotic chromosome segregation
homologous chromosome segregation
isotype switching
negative regulation of mitotic recombination
oogenesis
meiotic spindle midzone assembly
cellular response to organic cyclic compound
cell cycle
meiotic telomere clustering
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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