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MLK3 Polyclonal Antibody

MLK3 Antibody in Immunocytochemistry (ICC/IF)

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MLK3 Antibody (11996-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLK3 antibody (Product # 11996-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MLK3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

11996-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MLK3 Fusion Protein Ag2636 (1-379 aa encoded by BC011263)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEPLKSLFL KSPLGSWNGS GSGGGGGGGG GRPEGSPKAA GYANPVWTAL FDYEPSGQDE LALRKGDRVE VLSRDAAISG DEGWWAGQVG GQVGIFPSNY VSRGGGPPPC EVASFQELRL EEVIGIGGFG KVYRGSWRGE LVAVKAARQD PDEDISVTAE SVRQEARLFA MLAHPNIIAL KAVCLEEPNL CLVMEYAAGG PLSRALAGRR VPPHVLVNWA VQIARGMHYL HCEALVPVIH RDLKSNNILL LQPIESDDME HKTLKITDFG LAREWHKTTQ MSAAGTYAWM APEVIKASTF SKGSDVWSFG VLLWELLTGE VPYRGIDCLA VAYGVAVNKL TLPIPSTCPE PFAQLMADCW AQDPHRRPDF ASILQQLEAL (1-379 aa encoded by BC011263)

Target Information

MAP3K11 (MLK3) is a serine/threonine kinase that activates both the p38 MAP kinase and c-Jun kinase pathways. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy.

Acta pharmacologica Sinica

Huang J,Liu Y,Chen JX,Lu XY,Zhu WJ,Qin L,Xun ZX,Zheng QY,Li EM,Sun N,Xu C,Chen HY

Sat Jan 01 00:00:00 UTC 2022

Silence of MLK3 alleviates lipopolysaccharide-induced lung epithelial cell injury via inhibiting p53-mediated ferroptosis.

Journal of molecular histology

Chen X,Qi G,Fang F,Miao Y,Wang L

Fri Apr 01 00:00:00 UTC 2022

Xinyang Tablet inhibits MLK3-mediated pyroptosis to attenuate inflammation and cardiac dysfunction in pressure overload.

Journal of ethnopharmacology

Wang J,Deng B,Liu J,Liu Q,Guo Y,Yang Z,Fang C,Lu L,Chen Z,Xian S,Wang L,Huang Y

Mon Jun 28 00:00:00 UTC 2021

Frontiers in physiology

Ying C,Dai J,Fan G,Zhou Z,Gan T,Zhang Y,Song Y,Zhou X

Fri Jul 02 00:00:00 UTC 2021

Frontiers in physiology

Ying C,Dai J,Fan G,Zhou Z,Gan T,Zhang Y,Song Y,Zhou X

Fri Jul 02 00:00:00 UTC 2021

Frontiers in physiology

Ying C,Dai J,Fan G,Zhou Z,Gan T,Zhang Y,Song Y,Zhou X

Fri Jul 02 00:00:00 UTC 2021

Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload.

Cell death & disease

Wang J,Deng B,Liu Q,Huang Y,Chen W,Li J,Zhou Z,Zhang L,Liang B,He J,Chen Z,Yan C,Yang Z,Xian S,Wang L

Fri Jul 24 00:00:00 UTC 2020

Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.

International journal of molecular medicine

Yin M,Shen Z,Yang L,Zheng W,Song H

Wed May 01 00:00:00 UTC 2019

High-Throughput Screening Identifies Mixed-Lineage Kinase 3 as a Key Host Regulatory Factor in Zika Virus Infection.

Journal of virology

Xu H,Cheng M,Chi X,Liu X,Zhou J,Lin T,Yang W

Sun Sep 15 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: MAP3K11; MGC17114; mitogen activated protein kinase kinase kinase 11; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; protein-tyrosine kinase PTK1; SH3 domain-containing proline-rich kinase; Src-homology 3 domain-containing proline-rich kinase

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Gene Aliases: 2610017K16Rik; MAP3K11; MEKK11; MLK-3; MLK3; PTK1; RHOE; SPRK

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UniProt ID: (Human) Q16584, (Rat) Q66HA1, (Mouse) Q80XI6

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Entrez Gene ID: (Human) 4296, (Rat) 309168, (Mouse) 26403

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Function(s)

protein kinase activity protein serine/threonine kinase activity JUN kinase kinase kinase activity protein binding ATP binding mitogen-activated protein kinase kinase binding mitogen-activated protein kinase kinase kinase binding identical protein binding protein homodimerization activity Rac GTPase binding MAP kinase kinase kinase activity protein tyrosine kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

activation of MAPK activity protein phosphorylation microtubule-based process JNK cascade activation of JNKK activity activation of JUN kinase activity cell death cell proliferation positive regulation of JUN kinase activity positive regulation of neuron apoptotic process positive regulation of JNK cascade protein autophosphorylation peptidyl-tyrosine phosphorylation positive regulation of apoptotic process phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MLK3 Polyclonal Antibody

MLK3 Antibody (11996-1-AP) in ICC/IF

Product Details

11996-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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