Invitrogen

MLKL Polyclonal Antibody

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MLKL Antibody (PA5-34733) in WB

Western blot was performed using MLKL Polyclonal Antibody (Product # PA5-34733) and a 54kDa band corresponding to MLKL was observed across cell lines tested. Membrane enriched cell extracts (30 µg lysate) of HT-29 (Lane 1), THP-1 (Lane 2), U-937 (Lane 3) and HeLa (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:20... View More

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MLKL Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with MLKL siRNA and decrease in signal intensity was observed in western blot application using Anti-MLKL Polyclonal Antibody (Product # PA5-34733). {KD}

Product Details

PA5-34733

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MLKL. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.52 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2552085

Product Specific Information

Recommended positive controls: THP-1, Jurkat, HCT116, PC-3.

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The gp130/STAT3-endoplasmic reticulum stress axis regulates hepatocyte necroptosis in acute liver injury.

Croatian medical journal

Li X,Wang J,Li Y,He W,Cheng QJ,Liu X,Xu DL,Jiang ZG,Xiao X,He YH

Published figure using MLKL polyclonal antibody (Product # PA5-34733) in Western Blot

Fri Jun 30 00:00:00 UTC 2023

Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis.

BMC microbiology

Jeon YJ,Gil CH,Won J,Jo A,Kim HJ

Published figure using MLKL polyclonal antibody (Product # PA5-34733) in Western Blot

Fri May 26 00:00:00 UTC 2023

Intracellular alpha-fetoprotein mitigates hepatocyte apoptosis and necroptosis by inhibiting endoplasmic reticulum stress.

World journal of gastroenterology

Chen YF,Liu SY,Cheng QJ,Wang YJ,Chen S,Zhou YY,Liu X,Jiang ZG,Zhong WW,He YH

PA5-34733 was used in Western Blotting to investigate whether and how AFP could regulate ER stress and hepatocyte injury.

Thu Jul 14 00:00:00 UTC 2022

Tumor necrosis factor is a necroptosis-associated alarmin.

Frontiers in immunology

Pinci F,Gaidt MM,Jung C,Nagl D,Kuut G,Hornung V

PA5-34733 was used in Immunoprecipitation, Western Blot to demonstrate that lytic cell death, particularly necroptosis, is a critical trigger for TNF release and thus qualify TNF as a necroptosis-associated alarmin.

Fri Dec 30 00:00:00 UTC 2022

Downregulation of RIP3 Improves the Protective Effect of ATF6 in an Acute Liver Injury Model.

BioMed research international

Huang MY,Wan DW,Deng J,Guo WJ,Huang Y,Chen H,Xu DL,Jiang ZG,Xue Y,He YH

PA5-34733 was used in Western Blotting to investigate the impact of Activating transcription factor 6 on receptor-interacting protein 3 expression, and its role in hepatocyte necroptosis in an acute liver injury model.

Fri Jan 21 00:00:00 UTC 2022

Downregulation of RIP3 Improves the Protective Effect of ATF6 in an Acute Liver Injury Model.

BioMed research international

Huang MY,Wan DW,Deng J,Guo WJ,Huang Y,Chen H,Xu DL,Jiang ZG,Xue Y,He YH

Fri Jan 21 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: hMLKL; Mixed lineage kinase domain-like protein; MLKL

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Gene Aliases: hMLKL; MLKL

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UniProt ID: (Human) Q8NB16

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Entrez Gene ID: (Human) 197259

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Function(s)

protein kinase activity protein binding ATP binding protein kinase binding protein complex binding

Process(es)

protein phosphorylation protein homotrimerization necroptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MLKL Polyclonal Antibody

MLKL Antibody (PA5-34733) in WB

Product Details

PA5-34733

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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