Invitrogen

MLKL Polyclonal Antibody

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MLKL Antibody (PA5-43960) in WB

Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), SW480 (Lane 2), U-937 (Lane 3), HT-29 (Lane 4), tissue extracts of Mouse Brain (Lane 5) and Mouse Liver (Lane 6). The blot was probed with Anti-MLKL Polyclonal Antibody (Product # PA5-43960, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 55 kDa band corresponding to MLKL was observed across all the cell ... View More

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MLKL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of MLKL was observed in Mouse Liver in comparison to Mouse Brain using product (catalog # PA5-43960) in western blot. A non-specific band at approximately 110 kDa was observed in Mouse Brain tissue lysate. {RE}

Product Details

PA5-43960

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human MLKL (aa 107-156).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2606606

Product Specific Information

Peptide sequence: DVWKELSLLL QVEQRMPVSP ISQGASWAQE DQQDADEDRR AFQMLRRDNE

Sequence homology: Cow: 85%; Horse: 91%; Human: 100%; Rat: 90%

Target Information

The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Serum amyloid A3 is required for caerulein-induced acute pancreatitis through induction of RIP3-dependent necroptosis.

Immunology and cell biology

Yang X,Li R,Xu L,Qian F,Sun L

PA5-43960 was used in Western Blotting to evaluate the relationship of serum amyloid A and acute pancreatitis in mice.

Fri Jan 01 00:00:00 UTC 2021

Serum amyloid A3 is required for caerulein-induced acute pancreatitis through induction of RIP3-dependent necroptosis.

Immunology and cell biology

Yang X,Li R,Xu L,Qian F,Sun L

PA5-43960 was used in Western Blotting to evaluate the relationship of serum amyloid A and acute pancreatitis in mice.

Fri Jan 01 00:00:00 UTC 2021

Pomelo peel oil suppresses TNF-α-induced necroptosis and cerebral ischaemia-reperfusion injury in a rat model of cardiac arrest.

Pharmaceutical biology

Wang W,Xie L,Zou X,Hu W,Tian X,Zhao G,Chen M

PA5-43960 was used in Western Blot to investigate the effect of Pomelo peel oil tumor necrosis factor-α (TNF-α)-induced necroptosis in cerebral ischemia-reperfusion injury after cardiac arrest.

Wed Dec 01 00:00:00 UTC 2021

Pomelo peel oil suppresses TNF-α-induced necroptosis and cerebral ischaemia-reperfusion injury in a rat model of cardiac arrest.

Pharmaceutical biology

Wang W,Xie L,Zou X,Hu W,Tian X,Zhao G,Chen M

PA5-43960 was used in Western Blot to investigate the effect of Pomelo peel oil tumor necrosis factor-α (TNF-α)-induced necroptosis in cerebral ischemia-reperfusion injury after cardiac arrest.

Wed Dec 01 00:00:00 UTC 2021

Cell death inhibitors protect against brain damage caused by cardiac ischemia/reperfusion injury.

Cell death discovery

Liao S,Apaijai N,Luo Y,Wu J,Chunchai T,Singhanat K,Arunsak B,Benjanuwattra J,Chattipakorn N,Chattipakorn SC

PA5-43960 was used in Western Blotting to show that cell death inhibitors prevented hippocampal dendritic spine loss caused by cardiac I/R injury through different mechanisms.

Sat Oct 23 00:00:00 UTC 2021

Cell death inhibitors protect against brain damage caused by cardiac ischemia/reperfusion injury.

Cell death discovery

Liao S,Apaijai N,Luo Y,Wu J,Chunchai T,Singhanat K,Arunsak B,Benjanuwattra J,Chattipakorn N,Chattipakorn SC

PA5-43960 was used in Western Blotting to show that cell death inhibitors prevented hippocampal dendritic spine loss caused by cardiac I/R injury through different mechanisms.

Sat Oct 23 00:00:00 UTC 2021

Would Colloidal Gold Nanocarriers Present An Effective Diagnosis Or Treatment For Ischemic Stroke?

International journal of nanomedicine

Amani H,Mostafavi E,Alebouyeh MR,Arzaghi H,Akbarzadeh A,Pazoki-Toroudi H,Webster TJ

PA5-43960 was used in Western Blotting to evaluate OX26-PEG-coated gold nanoparticles (OX26@GNPs) as a novel targeted nanoparticulate system on cell survival after ischemic stroke.

Mon Dec 16 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: hMLKL; Mixed lineage kinase domain-like protein; MLKL

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Gene Aliases: hMLKL; MLKL

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UniProt ID: (Human) Q8NB16

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Entrez Gene ID: (Human) 197259

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Function(s)

protein kinase activity protein binding ATP binding protein kinase binding protein complex binding

Process(es)

protein phosphorylation protein homotrimerization necroptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MLKL Polyclonal Antibody

MLKL Antibody (PA5-43960) in WB

Product Details

PA5-43960

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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