Proteintech

MLXIP Polyclonal Antibody

MLXIP Antibody in Immunocytochemistry (ICC/IF)

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MLXIP Antibody (13614-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using MLXIP antibody 13614-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

MLXIP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MLXIP Antibody in Immunoprecipitation (IP)

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Product Details

13614-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

ChIP assay (ChIP)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MLXIP Fusion Protein Ag4519 (1-217 aa encoded by BC039704)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody could recognize isoform1 (~110 kDa) and isoform3(~69 kDa) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm.

Immunogen sequence: MDGQGCEHT SRTEDPFIQP TDFGPSEPPL SVPPPISPVL PLVPPPATAL NPPAPPTFHQ PQKFAGVNKA PSVITHTASA TLTHDAPATT FSQSQGLVIT THHPAPSAAP CGLALSPVTR PPQPRLTFVH PKPVSLTGGR PKQPHKIVPA PKPEPVSLVL KNARIAPGEP GGETQCGAPP DPEGCFPIPK AFKLVTTTTT LVCTCMRTHI HLNETKVS (1-217 aa encoded by BC039704)

Target Information

This gene encodes a protein that functions as part of a heterodimer to activate transcription. The encoded protein forms a heterodimer with Max-like protein X (MLX) and is involved in the regulation of genes in response to cellular glucose levels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.

Gastroenterology

Lu Y,Li Y,Liu Q,Tian N,Du P,Zhu F,Han Y,Liu X,Liu X,Peng X,Wang X,Wu Y,Tong L,Li Y,Zhu Y,Wu L,Zhang P,Xu Y,Chen H,Li B,Tong X

Sun Aug 01 00:00:00 UTC 2021

MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.

Gastroenterology

Lu Y,Li Y,Liu Q,Tian N,Du P,Zhu F,Han Y,Liu X,Liu X,Peng X,Wang X,Wu Y,Tong L,Li Y,Zhu Y,Wu L,Zhang P,Xu Y,Chen H,Li B,Tong X

Sun Aug 01 00:00:00 UTC 2021

MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.

Gastroenterology

Lu Y,Li Y,Liu Q,Tian N,Du P,Zhu F,Han Y,Liu X,Liu X,Peng X,Wang X,Wu Y,Tong L,Li Y,Zhu Y,Wu L,Zhang P,Xu Y,Chen H,Li B,Tong X

Sun Aug 01 00:00:00 UTC 2021

MondoA Is Required for Normal Myogenesis and Regulation of the Skeletal Muscle Glycogen Content in Mice.

Diabetes & metabolism journal

Ran H,Lu Y,Zhang Q,Hu Q,Zhao J,Wang K,Tong X,Su Q

Sat May 01 00:00:00 UTC 2021

The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.

PLoS biology

Carroll PA,Freie BW,Cheng PF,Kasinathan S,Gu H,Hedrich T,Dowdle JA,Venkataramani V,Ramani V,Wu X,Raftery D,Shendure J,Ayer DE,Muller CH,Eisenman RN

Fri Oct 01 00:00:00 UTC 2021

The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.

PLoS biology

Carroll PA,Freie BW,Cheng PF,Kasinathan S,Gu H,Hedrich T,Dowdle JA,Venkataramani V,Ramani V,Wu X,Raftery D,Shendure J,Ayer DE,Muller CH,Eisenman RN

Fri Oct 01 00:00:00 UTC 2021

Protein synthesis inhibitors stimulate MondoA transcriptional activity by driving an accumulation of glucose 6-phosphate.

Cancer & metabolism

Wilde BR,Kaadige MR,Guillen KP,Butterfield A,Welm BE,Ayer DE

Fri Dec 04 00:00:00 UTC 2020

Dl-3-n-Butylphthalide Inhibits NLRP3 Inflammasome and Mitigates Alzheimer's-Like Pathology via Nrf2-TXNIP-TrX Axis.

Antioxidants & redox signaling

Wang CY,Xu Y,Wang X,Guo C,Wang T,Wang ZY

Wed Apr 10 00:00:00 UTC 2019

Dl-3-n-Butylphthalide Inhibits NLRP3 Inflammasome and Mitigates Alzheimer's-Like Pathology via Nrf2-TXNIP-TrX Axis.

Antioxidants & redox signaling

Wang CY,Xu Y,Wang X,Guo C,Wang T,Wang ZY

Wed Apr 10 00:00:00 UTC 2019

Cellular acidosis triggers human MondoA transcriptional activity by driving mitochondrial ATP production.

eLife

Wilde BR,Ye Z,Lim TY,Ayer DE

Tue Feb 05 00:00:00 UTC 2019

MondoA drives muscle lipid accumulation and insulin resistance.

JCI insight

Ahn B,Wan S,Jaiswal N,Vega RB,Ayer DE,Titchenell PM,Han X,Won KJ,Kelly DP

Tue Jul 09 00:00:00 UTC 2019

Nature neuroscience

Chow HM,Shi M,Cheng A,Gao Y,Chen G,Song X,So RWL,Zhang J,Herrup K

Fri Nov 01 00:00:00 UTC 2019

MondoA Is an Essential Glucose-Responsive Transcription Factor in Human Pancreatic β-Cells.

Diabetes

Richards P,Rachdi L,Oshima M,Marchetti P,Bugliani M,Armanet M,Postic C,Guilmeau S,Scharfmann R

Thu Mar 01 00:00:00 UTC 2018

MondoA Is an Essential Glucose-Responsive Transcription Factor in Human Pancreatic β-Cells.

Diabetes

Richards P,Rachdi L,Oshima M,Marchetti P,Bugliani M,Armanet M,Postic C,Guilmeau S,Scharfmann R

Thu Mar 01 00:00:00 UTC 2018

BRCA1 deficiency sensitizes breast cancer cells to bromodomain and extra-terminal domain (BET) inhibition.

Oncogene

Zhang B,Lyu J,Liu Y,Wu C,Yang EJ,Pardeshi L,Tan K,Wong KH,Chen Q,Xu X,Deng CX,Shim JS

Sat Dec 01 00:00:00 UTC 2018

BRCA1 deficiency sensitizes breast cancer cells to bromodomain and extra-terminal domain (BET) inhibition.

Oncogene

Zhang B,Lyu J,Liu Y,Wu C,Yang EJ,Pardeshi L,Tan K,Wong KH,Chen Q,Xu X,Deng CX,Shim JS

Sat Dec 01 00:00:00 UTC 2018

Ras Suppresses TXNIP Expression by Restricting Ribosome Translocation.

Molecular and cellular biology

Ye Z,Ayer DE

Mon Oct 15 00:00:00 UTC 2018

Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.

Cancer cell

Carroll PA,Diolaiti D,McFerrin L,Gu H,Djukovic D,Du J,Cheng PF,Anderson S,Ulrich M,Hurley JB,Raftery D,Ayer DE,Eisenman RN

Mon Feb 09 00:00:00 UTC 2015

Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.

Cancer cell

Carroll PA,Diolaiti D,McFerrin L,Gu H,Djukovic D,Du J,Cheng PF,Anderson S,Ulrich M,Hurley JB,Raftery D,Ayer DE,Eisenman RN

Mon Feb 09 00:00:00 UTC 2015

Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.

Proceedings of the National Academy of Sciences of the United States of America

Shen L,O'Shea JM,Kaadige MR,Cunha S,Wilde BR,Cohen AL,Welm AL,Ayer DE

Tue Apr 28 00:00:00 UTC 2015

Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.

Proceedings of the National Academy of Sciences of the United States of America

Shen L,O'Shea JM,Kaadige MR,Cunha S,Wilde BR,Cohen AL,Welm AL,Ayer DE

Tue Apr 28 00:00:00 UTC 2015

Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.

PLoS pathogens

Sanchez EL,Carroll PA,Thalhofer AB,Lagunoff M

Wed Jul 01 00:00:00 UTC 2015

Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.

PLoS pathogens

Sanchez EL,Carroll PA,Thalhofer AB,Lagunoff M

Wed Jul 01 00:00:00 UTC 2015

MondoA-Mlx transcriptional activity is limited by mTOR-MondoA interaction.

Molecular and cellular biology

Kaadige MR,Yang J,Wilde BR,Ayer DE

Thu Jan 01 00:00:00 UTC 2015

MondoA-Mlx transcriptional activity is limited by mTOR-MondoA interaction.

Molecular and cellular biology

Kaadige MR,Yang J,Wilde BR,Ayer DE

Thu Jan 01 00:00:00 UTC 2015

MondoA-Mlx transcriptional activity is limited by mTOR-MondoA interaction.

Molecular and cellular biology

Kaadige MR,Yang J,Wilde BR,Ayer DE

Thu Jan 01 00:00:00 UTC 2015

MondoA-Mlx transcriptional activity is limited by mTOR-MondoA interaction.

Molecular and cellular biology

Kaadige MR,Yang J,Wilde BR,Ayer DE

Thu Jan 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: bHLHe36; Class E basic helix-loop-helix protein 36; MLX interacting protein; Mlx interactor; MLX-interacting protein; MLXIP; Transcriptional activator MondoA

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Gene Aliases: AW228700; BHLHE36; KIAA0867; MIR; MLXIP; MONDOA; RGD1560774

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UniProt ID: (Human) Q9HAP2, (Mouse) Q2VPU4

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Entrez Gene ID: (Human) 22877, (Rat) 304479, (Mouse) 208104

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Function(s)

transcription factor activity, transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding protein dimerization activity protein binding

Process(es)

regulation of transcription from RNA polymerase II promoter nucleocytoplasmic transport regulation of carbohydrate metabolic process by regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MLXIP Polyclonal Antibody

MLXIP Antibody (13614-1-AP) in ICC/IF

Product Details

13614-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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